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In the present analysis marker assisted electrophoretic protein banding profiling was analyzed for five pulses which are sold more in the market and consumed by the people at large scale. The electrophoretic protein profiling was done in the samples of Chana dal (Cicer arietinum, 2n=16), Moong dal (Vigna radiata, 2n=14), Masoor dal (Lens culinaris, 2n=14), Tuvar dal (Cajanus indicus, 2n=22), and Udid dal (Vigna angularis, 2n=22). The protein extraction was done from selected seed samples and the crude protein content was estimated by Lowry’s method. SDS-PAGE for the proteins was carried out. The Standard protein marker showed the existence of total 4 bands, with RF range from 0.16 to 0.74 and molecular weight in the range of 15 to 66 kDa. Cicer arietinum showed 5 bands with RF range, 0.094 to 0.64 and molecular weight in the range of 09 to 63 kDa, Vigna radiata reveals 8 bands with RF value ranging from 0.072 to 0.78 and molecular weight in the range of 07 to 75 kDa, Lens culinaris showed 7 bands with RF, 0.10 to 0.78 and molecular weight in the range of 10 to75 kDa, Cajanus indicus showed 5 bands with RF 0.33 to 0.76 kDa, and molecular weight in the range 32 to 73 kDa and Vigna angularis showed 6 bands, with RF 0.14 to 0.79 and molecular weight in the range 14 to76 kDa respectively. Present analysis indicated Cicer arietinum has highest amount of proteins among studied samples. The studies on banding pattern reveals the insight regarding marker assisted identification and location of various protein complexes and the relation between other legume species. In India, leguminous pulses serve as major source for protein intake. The present work was undertaken due to consumption of essential legume protein components and its importance in our daily life.

Keywords: Electrophoresis, protein profile, SDS-PAGE, Leguminous Pulses.