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Antimicrobial peptides (AMPs) or bacteriocins consist of molecules that act on the defence systems
of numerous organisms. These compounds have become extremely significant due to the increasing
resistance of microorganisms to common antibiotics. In this work, selective media was used for
isolation of Lactobacillus species producing bacteriocins. Optimization was carried out for physical
and media conditions. Optimized conditions were found to be temperature 37ºC, neutral pH, lactose
at 2.0% concentration and ammonium nitrate at 2.5%. Under these optimized conditions enhanced
production of bacteriocin was observed. Further produced bacteriocin was subjected to mass
spectroscopy and presence of multiple bacteriocins was observed.
Keywords:Antimicrobial, Bacteriocin, Lactobacillus, Optimization.