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Aspergillus flavus is a fungus that often contaminates concentrate feed. This fungus is able to produce
aflatoxin B1, which is harmful to livestock and humans. The aim of this study is to determine the potential
of the filtrate of Trichoderma asperillum TKD in reducing aflatoxin B1 produced by Aspergillus flavus
FNCC 6109. The test is done by formulating concentrate feed with filtrate concentrations of 20 %; 40 %
and 60 %. The level of aflatoxin B1 is determined by ELISA technique (Enzyme Linked Immuno Sorbent
Assay). The characterization of secondary metabolites in the filtrate is analyzed by using GCMS. The
results showed that 60 % of filtrate concentration was able to reduce aflatoxin B1 by 47.84 % which is
significantly different (P≤0,05) to other treatments. The secondary metabolites contained in T. asperillum
TKD filtrate are Butane, 1,1 - oxybis- (CAS) n - Butyl ether; Butane, 1,1 - dibutoxy - ; Pentansaeure,
2,24,4 - Tetramethyl; 1 - Tetradecene; Phenol, 2,4 - bis (1,1 - dimethylethyl- (CAS) 2,4; 1- Hexadecane
(CAS) Cetene; 1 - Octadecene (CAS) alpha - Octadecene ; hexadecanoic acid , methyl ester (CAS)
Methyl, n - Tetracosano - 1 , 4 - Heptanone (CAS) GBL; 1 - Dodecanol (CAS) n - Dodecanol and Behenic
alcohol.
Keywords: aflatoxin B1, filtrate, T. asperillum TKD, concentrate.