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Red pigmented bacterial strain isolated from the soil sample of Kharaghoda area, Gujarat was used for
the present study. This halophile, Gram negative bacteria was identified as a Serratia marcescens strain
by 16s rRNA gene sequencing. This bacterium showed salt tolerance up to 12% Nacl. The gene sequence
was submitted to NCBI and it is released by named Serratia marcescens KH1R KM035849. The highest
extraction of this pigment was yielded in 2:1= 85% Methanol: Acetone mixture among different
concentration of different solvents. The Rf value of the extracted pigment was 0.64-0.96 in TLC method.
The maximum absorption spectrum was observed at 535 nm by UV-Visible Spectroscopy. This pigment
exhibited antibacterial activity against B.cerus, S.aureus and E.coli with inhibition zone 12 mm, 07 mm
and 06 mm respectively. This pigment was showing antioxidant activity confirmed by DPPH reduction
method. The Serratia marcescens KH1R KM035849 strain is also siderophore producer.
Keywords: Halophile, Serratia marcescens, Prodigiosin, Pigment extraction.