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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2014 , Volume 2, Issue 6
Page No. : 12-26
Article doi: : http://dx.doi.org/10.18782
Effect of a dual inoculation with endomycorrhizae and Trichoderma harzianum on the growth of date palm seedlings
Fadoua Sghir1, Mohamed Chliyeh1, Jihane Touati1, Btissam Mouria1, Amina Ouazzani Touhami1, Abdelkarim Filali-Maltouf2, Cherkaoui El Modafar3, Abdelmajid Moukhli4, Rachid Benkirane1, and Allal Douira1*
1Laboratoire de Botanique et de Protection des Plantes, UFR de Mycologie, Département de Biologie, Faculté des Sciences BP. 133, Université Ibn Tofail, Kénitra, Maroc
2Laboratoire de Microbiologie et Biologie Moléculaire, Faculté des Sciences, Université Mohammed V Agdal, Av Ibn Batouta, BP 1014 Rabat, Maroc
3Laboratoire de Biotechnologie, Valorisation et Protection des Agroressources, Faculté des Sciences et Techniques Guéliz, B.P. 618, 40 000 Marrakech, Maroc
4UR, Amélioration génétique des plantes, Institut national de la Recherche agronomique F- 40 000 Marrakech, Maroc
*Corresponding Author E-mail: douiraallal@hotmail.com
ABSTRACT
The dual inoculation with endomycorrhizae and Trichoderma harzianum had a significant effect on the
growth of date palm plants. In fact, the average values of aerial fresh weight (38.99g), number of leaves
(6.7), the plant length (38cm), and the stem diameter (1.2 cm) of the plant inoculated with AMF and
Trichoderma harzianum, were higher than those inoculated only with AMF, respectively, 32g, 5.2 , 38 cm
et 1.2cm. or just with Trichoderma harzianum 30g, 3.75, 46cm et 0.8cm. by contrast, the mycorrhizal
intensity of roots inoculated only with AMF (82%) was higher than that of plants inoculated with AMF
and T. harzianum (60%). thus it seems that T. harzianum reduced root colonization by mycorrhizal fungi.
Otherwise, 25 endomycorrhizal species were isolated in the rhizosphere of the inoculated plant after nine
months of inoculation. These species belong to the genera Glomus (55%), Acaolospora (22%),
Scutellospora (12%), Entrophospora (5%) and Gigaspora(2%).
Keywords: Endomycorrhizae, AMF, Trichoderma harzianum, etc.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article:
Int. J. Pure App. Biosci. 2 (6): 12-26 (2014)
