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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2018 , Volume 6, Issue 4
Page No. : 134-140
Article doi: : http://dx.doi.org/10.18782/2320-7051.6616
In vitro Callus Induction in Guggul [Commiphora wightii (Arnott)]- An Endangered Medicinal Plant
Kumawat, S.*, Kumar, R., Shekhawat, K., Kumawat, K., Choudhary, R., Verma R.1 and Jakhar, M. L.2
1Research Scholar and 2Professor
Department of Plant Breeding and Genetics, SKN Agriculture University, Jobner
*Corresponding Author E-mail: kumawatswarnlata.sk@gmail.com
Received: 15.06.2018 | Revised: 22.07.2018 | Accepted: 29.07.2018
ABSTRACT
Leaf explant and nodal segment explants of guggul were placed on Murashige and Skoog Medium, supplemented with different concentration of cytokinins and auxins alone or in combination for callus induction. In leaf explants maximum callus induction was observed on a medium containing 2.0 mg/l 2, 4-D with 100 per cent frequency followed by 5.0 mg/l Kn+0.5 mg/l NAA with 100 per cent frequency. Whereas, maximum callus induction from nodal segment explant was observed on a medium containing 5.0 mg/l BAP+5.0 mg/l NAA with 100 per cent frequency. Key words: Guggul, Callus induction, Tissue culture, In vitro.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782/2320-7051.6616
Cite this article:
Kumawat, S., Kumar, R., Shekhawat, K., Kumawat, K., Choudhary, R., Verma, R. and Jakhar, M.L., In vitro Callus Induction in Guggul [Commiphora wightii (Arnott)]- An Endangered Medicinal Plant , Int. J. Pure App. Biosci. 6(4): 134-140 (2018). doi: http://dx.doi.org/10.18782/2320-7051.6616
