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Agriculture Important Microorganism (AIM) as well as Plant Growth Promoting Bacteria (PGPB) are considered a promising approach to replace the conventional agricultural practices, although plant growth-promoting rhizobacteria (PGPR) have been reported to influence plant growth, yield and nutrient uptake by an array of mechanisms, the specific character by which PGPR encourage plant growth, yield and nutrient uptake were limited to the expression of one or more of the character expressed at a given surroundings of plant–microbe interaction. Since they have been shown to affect plant nutrient-acquisition processes by influencing nutrient availability in the rhizosphere and those biochemical processes determining the uptake at root level of nitrogen (N), phosphorus (P), and iron (Fe), that represent the major constraints for crop productivity worldwide. We have isolated novel bacterial strains from the rhizosphere of Coriander (Coriandrum sativum L.) seed spice crop. Rhizospheric soil sample of seed spice coriander crop was collected from Digod, Kota district of Hadoti area in Rajasthan State, India. The optimum temperature, pH and EC of collected soil sample were found to be 29ºC and 7.7, 0.08 dS/m respectively. COR-3 bacterial isolated from coriander rhizosphere soil sample and have been characterized morphological and biochemical parameters. The COR-3 isolate exhibited capacity to produce siderophores and catalase, citrase enzymes and to solubilize phosphate. COR-3 identified by molecular tools and characterized for 16S rDNA nucleotide sequencing, the result indicated that Pseudomonas putida was identified. The morphological, biochemical and molecular studies showed that Pseudomonas putida has significant potential to increase the production and to uncover its efficacy as effective agriculture important microorganism (AIM).

Key words: Agriculture important microorganism, Pseudomonas putida, Coriander seed spice crop, 16S rDNA Sequencing, Phylogenetic study.