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One of the most important limiting factors of agriculture is plant diseases caused by various pathogens like fungi, bacteria, viruses and nematodes. Exclusive dependence on chemicals is leading to health issues and of late the problem of resistance in pathogens. RNA interference (RNAi) is a novel strategy for control of various pathogens through silencing of genes associated with pathogenesis. Different methods of introducing dsRNA in to cells are Agroinfiltration, Micro bombardment and Virus Induced Gene Silencing VIGS. History of RNAi and implications of RNAi technology in management of various plant pathogens like fungi, bacteria, viruses and nematodes are briefly reviewed.

Key words: RNA interference (RNAi)-History-Fungi-Bacteria-Viruses-Nematodes