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Plants are healthy and natural resource of life. In particular, medicinal plants are of great importance with endless therapeutic properties useful for curing various diseases with an advantage of being natural. The present study is to evaluate the qualitative estimation of phytochemicals and antimicrobial activity of ethyl acetate, benzene, hexane, chloroform, methanol extracts of leaves of Croton bonplandianum against bacteria such as Staphylococcus haemolyticus : MTCC 3383 and Salmonella enterica ser. typhi: MTCC 8767. The process was carried out by agar well diffusion method. The extracts were poured into the wells at different concentrations like 25mg/ml, 50mg/ml, 150mg/ml and 300mg/ml. After incubation zones of inhibition were observed. As the concentrations of extracts increased the activity also increased and thus the zone of inhibition too increased. Among five extracts zone of inhibition was best in ethyl acetate extract. . In case of Salmonella enterica ser. typhi: (MTCC 8767) the ethyl acetate extract (300 mg/ml) showed maximum zone of inhibition 20.6±1mm, while chloroform extract (25mg/ml) showed minimum zone of inhibition 11.0±00. In case of Staphylococcus haemolyticus: (MTCC 3383) the ethyl acetate extract (300 mg/ml) showed maximum zone of inhibition 18.0±1mm, while benzene extract (25mg/ml) showed minimum zone of inhibition 11.0±2.0.Hence Croton bonplandianum can be used in developing drugs and medicines against various activities of bacteria. Study has also been shown the presence of various phytochemical constituents such as alkaloid, tannin, saponin thiamine, ascorbic acid, phenolic content in the leaf of Croton bonplandianum.

Key words: Staphylococcus, Saponin thiamine, Ascorbic acid, Phenolic