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The enzymatic activity in the soil is mainly of microbial origin, being derived from intracellular, cell-associated or free enzymes. The present study on microbiological investigations, biochemical and enzymatic analysis of plants and pot experiments were carried out in the Department of Agricultural Microbiology and Bioenergy, PJTSAU, Rajendranagar, Hyderabad, on phosphate solubilizing bacteria and with these phosphate solubilizing bacteria (PSB) in association with Trichodema fungi different combinations of biofilms and coinoculations were prepared. The experiment was conducted in Mung bean crop. Three enzymes (Dehydrogenase, Phosphatase and Urease) activity was observed at all the three plant growth stages, and flowering stage being the highest for dehydrogenase (87.71 μg of TPF/g of soil/day), acidic phosphatase (130 μg p-nitro phenol/g of soil/day) and alkaline phosphatase (148 μg p-nitro phenol/g of soil/day) activity as a result of high persistency of microorganisms at this stage in T4 treatment. Since chemical fertilizers are not frequently used with Mungbean crop, use of PSB, biofilms, coinoculants as biofertilizers would help in improving better crop stand and crop yields.

Key words: Biofertilizers, Biofilms, Coinoculants, Enzymes.