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Postharvest pathogen Colletotrichum musae was isolated from diseased banana fruits. In vitro evaluation of botanicals against Colletotrichum musae revealed that highest per cent inhibition (77.96 %) of mycelial growth of C. musae was recorded in neem leaf extract followed by Garlic bulb extract (60.03 %), tulsi leaf extract (57.00 %). Least inhibition (5.67 %) was recorded in tridax leaf extract. Highest inhibition of spore germination of C. musae was recorded in garlic bulb extract (65.71 %), followed by tulsi leaf extract (61.31 %), neem leaf extract (57.16 %). Among the biocontrol agents, Bacillus subtilis recorded highest inhibition (84.45 %) followed by Pseudomonas fluorescens (79.30 %) and Trichoderma. viride (77.02 %). Hence, these botanicals and biocontrol agents can be effectively used as alternatives to chemicals.

Keywords: Pathogen, Tulsi, Neem, Biocontrol agents, Trichoderma