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To evaluate the analgesic, anti-inflammatory and anti-cancer activities of the ethanol extract of cartilage of
Aetobatus narinari in different experimental models in mice and rats. The dried cartilage was reduced to
fine powder and refluxed with ethanol. Analgesic activity of cartilage extract of Aetobatus narinari was
assessed in tail flick model in rats (n = 5), and acetic acid induced writhing in mice (n = 5), Antiinflammatory
activity was evaluated using carrageenan induced rat paw edema and the anti-cancer
activity (In-vitro dye exclusion method) was assessed in DAL cells bearing mice. The cartilage extract
exhibited 38.2 ± 4.33 and 22.6 ± 1.17 writhing and shown the protection of 50.52% and 70.73% at 200
and 400 mg·kg-1, respectively. The tail flick method revealed an increase in basal reaction time from 2.4
± 0.25 and 2.6 ± 0.40 sec at 0 min to 4.6 ± 0.51 and 6.4 ± 0.40 sec at 90 min at a dose of 200 and 400
mg·kg-1, respectively. The extract at 200 mg· kg-1 inhibited the edema volume by 70% with a mean
edema volume of 0.140 ± 0.006 at 120 min, whereas the crude ethanol cartilage extract at 400 mg·kg-1
concentration produced 80% inhibition of edema volume with a mean 0.120 ± 0.009 at 120 min. The
ethanol extract of Aetobatus narinari showed a significant cytotoxic activity to the tune of 88% at a
concentration of 200 μg in Dalton’s Ascites Lymphoma cells in vitro. The present investigation confirmed
the analgesic, anti-inflammatory and anticancer properties of Aetobatus narinari, and further
investigations are required to augment such potentials.
Keywords: Aetobatus narinari, Cartilage, Analgesic, Anti-inflammatory, Anticancer.