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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2017 , Volume 5, Issue 6
Page No. : 1389-1402
Article doi: : http://dx.doi.org/10.18782/2320-7051.5294
Designer Nucleases for Genome Editing in Plants: Different Approaches and Applications
Amardeep1*, Kisa Zaidi2, Shalu Sharma3 and Vikas Sharma4
1, 4Chaudhary Charan Singh University, Meerut-250 004, Uttar Pradesh, India 2Department of Biosciences, Shri Ram College, Muzaffarnagar, Uttar Pradesh, India 3College of Applied Education & Health Sciences (CAEHS), Meerut-250 001, Uttar Pradesh, India
*Corresponding Author E-mail: amar92home@gmail.com
Received: 23.07.2017 | Revised: 28.08.2017 | Accepted: 4.09.2017
ABSTRACT
Targeted genome editing using artificial nucleases has the potential to accelerate basic research as well as plant breeding by providing the means to modify genomes rapidly in a precise and predictable manner. Zinc-finger nucleases (ZFNs), transcription activator like effector nucleases (TALENs) and CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated proteins) system comprise a powerful class of tools that are redefining the boundaries of biological research. These chimeric nucleases enable a broad range of genetic modifications by inducing DNA double-strand breaks that stimulate error prone nonhomologous end joining or homology-directed repair at specific genomic locations. In this review, recent approaches and progress of artificial nuclease technology in plants has been summarized and discussed.
Key words: ZFNs, TALENs, CRISPR/Cas, NHEJ, HDR.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782/2320-7051.5294
Cite this article:
Amardeep, Zaidi, K., Sharma, S. and Sharma, V., Designer Nucleases for Genome Editing in Plants: Different Approaches and Applications, Int. J. Pure App. Biosci. 5(6): 1389-1402 (2017). doi: http://dx.doi.org/10.18782/2320-7051.5294
