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In the following study genomic DNA was extracted from eight Jasmine species. DNA fingerprinting was
performed by the random amplified polymorphic DNA (RAPD)-PCR. RAPD has been one of the most
commonly used molecular techniques to develop DNA markers since it does not require prior knowledge
of a DNA sequence. RAPD-PCR produced a spectrum of amplification products which are characteristics
of the selected Jasmine DNA. From the DNA fingerprinting, Dendrogram was constructed and genetic
similarity matrix were estimated which revealed variations between selected species of Jasmine. A total of
10 random primers were used for conducting the RAPD analysis. The primers were OPU5, OPU6,
OPU7, OPU8, OPU9, OPU10, OPU11, OPU12, OPU13, OPU14 and OPU15. Of these selected random
primers OPU8, OPU9, OPU10, OPU11, OPU12, produced clear banding patterns which were used for
constructing Dendrogram. OPU8 produced a total 55 bands ranging from 6-8, OPU9 produced a total 44
bands ranging from 2-8, OPU10 produced a total 33 bands ranging from 3-6, OPU11 produced a total
82 bands ranging from 9-12, OPU12 produced a total 61 bands ranging from 5-9. Most of the bands
were monomorphic with some polymorphic bands which can be used for marker development for these
jasmine species. The described approach holds great promise for genetic diversity polymorphism,
cultivar characterization and genetic population conservation of Jasmine species.
Keywords: Jasmine, DNA Fingerprinting, Dendrogram, RAPD, Genetic Diversity.