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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2014 , Volume 2, Issue 3
Page No. : 265-271
Article doi: : http://dx.doi.org/10.18782
Enzyme Activities of Bacterial Isolates from Iron Mine Areas of Barbil, Keonjhar District, Odisha, India
Debaraj Parida1, Santanu Kumar Jena, and Chandi Charan Rath2*
P.G. Deaprtment of Botany, North Orissa University, Baripada-757003, Odisha
1Department of Botany, Govt. Women’s College, Keonjhar 758001, Odisha, India
2Deputy Director (Technical), Biotechnology, Science and Technology Department, Govt. of Odisha,
Bhubaneswar -751001,Odisha, India
*Corresponding Author E-mail: chandicharanrath@yahoo.com
ABSTRACT
Microbial population and microbial activity are considered to be indicative measures of soil quality. In
the present investigation it was found that bacterial load ranged between1.04X104 to 7.8 X 105 CFUgm-1
soil and 4.7X 103 to 6X104 CFUgm-1soil of unexplored and explored iron mining areas respectively.
Whereas, phosphate solubilizing bacterial load was found to be very low, 3X102 to 5.9X103 CFUgm-1 of
soil. In toto 110 bacteria were isolated and identified as Bacillus sp. 64(58.2%), 4(3.6%) were
Pseudomonas sp., 2(1.81%) Sulphobaccilus sp., and one (0.9%) each from Ancyclobacter sp.,
Enterobacter sp., Acetobacter sp. and Geomicrococcus sp. It was found that 33(30%), and 39(33.5%) of
the isolates showed amylase and protease activity respectively. But, 24(21.8%) of the isolates showed
both amylase and protease activities. Only 4(3.63%) isolates showed phosphatase activity. Phosphate
solubilising activities of microbes in association with starch/complex polysaccharides and protein
degrading capabilities make them a suitable component to maintain soil fertility.
Keywords: Mining soil, Phosphatase, Amylase, Protease.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article:
Int. J. Pure App. Biosci. 2 (3): 265-271 (2014)
