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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2014 , Volume 2, Issue 3
Page No. : 203-206
Article doi: : http://dx.doi.org/10.18782
In vitro Evaluation of the Efficacy of Leaf and its Callus Extracts of Tinospora cordifolia (Willd.) Hook.F.Thoms on Pathogenic Fungi
Shanthi.V1* and R. Nelson2
1Department of Biology, College of Science, Eritrea Institute of Technology, Mai Nefhi,Eritrea, North East Africa
2Department of Botany, Government Arts College, Ariyalur. Tamil Nadu, India
*Corresponding Author E-mail: bioshanthi@gmail.com
ABSTRACT
The investigation is aimed to carry out the antifungal activities of ethanol extracts of leaf,stem, leaf
derived callus and stem derived callus of Tinospora cordifolia (Willd.) Hook.F.Thoms (Menispermaceae).
The leaf and stem segments of Tinospora cordifolia were cultured on MS medium supplemented with
auxins and cytokinins alone. In the case of leaf derived callus, maximum biomass was recorded on
medium containing 2,4-D, NAA and BAP combination while NAA and BAP combination showed good
response for callus induction in stem. The antimicrobial activities of ethanol extracts of leaf, stem, leaf
derived callus and stem derived callus were screened against ten fungal species using poisoned food
technique. All the tested extracts were bioactive with inhibition in germination of fungal spores.
Keywords: Tinospora cordifolia, callus culture, antifungal activity,MS medium, poisoned food technique.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article:
Int. J. Pure App. Biosci. 2 (3): 203-206 (2014)
