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The objective of this study was to find out the genetic relationship and variations among different
varieties of gold fishes using Random Amplified Polymorphic DNA (RAPD) amplification assay. High
degree of polymorphism was observed among the samples, suggesting the degree of genetic variability.
The low levels of genetic variation within-species are may be due to their limited migration and pair
fidelity mode of reproduction. The dendrogram obtained from RAPD by using two different primers OPA
11 and OPD 8 clearly depicts the genetic similarity and genetic variation within the gold fish species. The
data collected from this study will help in understanding their complex traits such as shape, size, colour,
morphological variations, tail and life span.
Keywords: Gold fish, RAPD, PCR, Genetic variations, Polymorphism, Dendrogram, Marker.