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The efficiency of cellulase produced by Aspergillus flavus (NFCCI 2703) in bioconversion viasolid
state fermentation using paper waste and bagasse as substrates was studied. Optimization parameters
and effect of co-inducers on production of FPase, CMCase, and cellobiose were assayed. Optimum
pH for paper waste was 4.5 and 5.5 for bagasse, 300C was optimum for both substrates, moisture content
was found to be 85% and 90% for bagasse and paper waste respectively. Sucrose was found to be best
co-inducer for bagasse and enhanced the activity by 21.91%, 11.11%, 11.77% of CMCase, FPase and
cellobiase respectively. Using paper waste as substrate the CMCase, FPase and cellobiase activities
were enhanced by 21.6%, 34.65%, 29.55%, respectively by cellulose. Bioconversion of paper waste
was 20% more as compared to bagasse though; higher concentrations of substrate and incubation
period inhibited the enzyme. This study reports that cellulase produced by A. flavus (NFCCI 2703)
was more effective in bioconversion of paper waste as compared to bagasse.
Keywords: Bioconversion, cellulase, solid state fermentation, baggase.