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In the present work the impact of Azotobacter sps. were studied on the garden soil to produce natural polymers. Due to stringent environment protection norms technologies are being directed towards the developments of non polluting biogreen materials. A biologically synthesized plastic polyhydroxyalkanoate has been attracting great interest owing to its properties similar to petroleum based plastics. Microorganism is producers of natural polymers and these microbial polymers are water soluble and have novel physical and chemical properties. Azotobacter sps was isolated from garden soil (IOS) using Ashby`s medium. Polysaccharide production was studied by using Garcia`s minimal medium as production medium in a benchtop bioreactor (HT infors). Harvested biomass was solvent extracted to obtain the polymer film, characterization of polymer was done using Gas chromatography. A clear polymer film was formed after chloroform extraction. Compounds observed in GCMS analysis of dried chloroform extract were Pthalic acid, isobutyl 4octyl ester (C20H30O4) 5.44%, n-Hexadecanoic acid (C16H32O2) 53.51% and Phytol(C20H40O) 5.28%.

Keywords: Biopolymers, Azotbactor sps., Biomass, Biodegradable plastic, GC-MS.