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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2014 , Volume 2, Issue 2
Page No. : 213-220
Article doi: : http://dx.doi.org/10.18782
Production and Partial Characterization of Protease from Staphylococcus sp. Isolated from the Mud Crab, Scylla serrata
Anandkumar1, Ramanadevi, V.1, Ajithkumar, T. T.2, Lipton, A. P.3 and Thangaraj, M.1*
1CAS in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai, Tamilnadu- 608 502
2National Bureau of Fish Genetic Resources, Lucknow, U.P-226 002
3VRC of CMFRI, Vizhinjam, Thiruvananthapuram, Kerala-695 521
*Corresponding Author E-mail: coralholder@yahoo.com
ABSTRACT
Proteases are generally used in detergents, food industries, leather, meat processing, cheese making,
silver recovery from photographic film, production of digestive and certain medical treatments of
inflammation and virulent wounds. The present study was aimed to isolate the protease producing
bacteria from the gut of mud crab (Scylla serrata) and optimize the production and find the molecular
weight. The potent protease producing bacteria was identified as Staphylococcus sp. by conventional and
molecular methods. The optimum condition for protease production in liquid media was: Temperature,
45oC; Salinity, 3% and pH 7.0. The best nutrient source for high protease production in liquid media was
Mannitol as carbon source and Potassium nitrate as nitrogen source. The specific activity of crude
enzyme and partially purified was 207.03 and 315.69 U/mg respectively. The molecular weight of the
protease enzyme produced by the crab gut associated Staphylococcus sp. was determined as 25 KDa.
Keywords: Mud crab, Scylla serrata, Gut flora, Staphylococcus, Protease.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article:
Int. J. Pure App. Biosci. 2 (2): 213-220 (2014)
