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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2014 , Volume 2, Issue 2
Page No. : 181-186
Article doi: : http://dx.doi.org/10.18782
An Efficient Callus Induction from Phyllanthus debilis Klein Ex Willd- A Wild Medicinal Plant of Eastern Ghats, India
Malayaman1. V, Ghouse Basha1. M* and Amzad Basha Kolar2
1P.G. & Research Department of Botany, Jamal Mohamed College (Autonomous), Tiruchirappalli - 620 020,
Tamil Nadu, India.
2Department of Molecular Biology, Bangalore University, Bangalore-560056, Karnataka, India.
*Corresponding Author E-mail: drghobashjmc@gmail.com
ABSTRACT
An efficient tissue culture protocol is developed for the medicinally persuasive plant Phyllanthus debilis
Klein ex Willd. (Euphorbiaceae) using leaf and internodal explants on Murashige and Skoog’s medium
with different combination and concentrations of growth regulators. The leaf segments produced
maximum callus induction on 45 days (82.5%) when MS medium was fortified with BAP (3.5 mg/l), NAA
(2.5 mg/l), 2, 4-D (0.5 mg/l) and the best response was observed on internodal callusing (80%) when the
MS medium contained 3.0 mg/l BAP, 2.0 mg/l NAA and 0.5 mg/l 2, 4-D. It was observed that the explants
produced scanty callus when the concentration of the hormones are low.
Keywords: Phyllanthus debilis, MS medium, Callus induction, Plant growth regulators.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article:
Int. J. Pure App. Biosci. 2 (2): 181-186 (2014)
