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An efficient, rapid and large-scale in vitro clonal propagation of the valuable medicinal herb, Eclipta
alba (Asteraceae) using seed was designed. The type of medium used, plant growth regulators markedly
influenced in vitro propagation of E. alba. The in vitro plantlet production system was investigated on
Murashige and Skoog (MS) medium with the synergistic combination of BAP (2 mg/L) and NAA(1 mg/L).
This composition of the medium influenced rapid plant growth .Sub culturing of the cut segments of the
grown plant on similar medium enabled continuous production of healthy shoots with similar frequency.
Each segment of the plantlet proliferated and grew into healthy plants having perfect rooting and
shooting. Micro propagated plants acclimatized in a mixture containing garden soil, and vermicompost
(2:1).The established plant in the pot were uniform and identical to the donor plant with respect to
growth characteristics. The method seems to be efficient which has been performed in two steps. The first
step being cultivation of plant from seed which can be suitably used for genetic manipulation of the plant
for useful purpose. The second step can be used for mass propagation. The method seems to be efficient
as both the step can be performed on the same composition of the medium.
Keywords: Eclipta alba, Seed, Plant as ex-plant, Propagation, Acclimatization.