Archives

Separation and purification of superoxide dismutase from pig blood and its activity protective agents
including metal ions and carbohydrates were explored. The results exhibited that specific activity of SOD
was 6594.55U/mg after it was purified by Sephdex G-100. There were three kinds of metal ions in terms
of effects on the activity of SOD. Na+, K+ and Zn2+ could promote activity of SOD with the increase of
their concentrations all the time. Ba2+、Ca2+、Cu2+ could also enhance activity of SOD, but it attenuated
with the increase of their concentrations. Mg2+、Fe2+、Al3+ raised activity of SOD at lower low
concentration but decreased it at higher concentration. All carbohydrates could slow down the reducing
rate of relative enzyme activity and had stabilizing effects on SOD except for D-fructose and sucrose with
higher concentration (0.1mol/L) and D-mannose with lower concentration (0.1mol/L). D-fructose
(0.5mol/L), D-galactose (0.2mol/L), D-arabinose (0.2mol/L) and D-glucose (0.2mol/L) could greatly
promote SOD activity all the time. These results suggested that some metal ions and carbohydrates can
be added to functional food or other products contained in SOD as active components for stabilizing or
promoting the activity of SOD.
Keyword：superoxide dismutase, metal ions; carbohydrate; enzyme activity.