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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2014 , Volume 2, Issue 2
Page No. : 28-38
Article doi: : http://dx.doi.org/10.18782
Comparative study of pathogenicity tests for Verticillium dahliae and Phytophthora palmivora causing wilt and decline of olive tree (Olea europaea L.)
Mohamed Chliyeh1, Youssef Rhimini1, Karima Selmaoui1, Amina Ouazzani Touhami1, Abdelkarim Filali-Maltouf2, Cherkaoui El Modafar3, Abdelmajid Moukhli4, Ahmed Oukabli5, Rachid Benkirane1 and Allal Douira1*
1Laboratoire de Botanique et de Protection des Plantes, UFR de Mycologie, Département de Biologie, Faculté des sciences BP, 133, Université Ibn Tofail, Kenitra, Maroc
2Laboratoire de Microbiologie et Biologie Moléculaire, Faculté des Sciences, Université Mohammed V Agdal, Av. Ibn Batouta, BP 1014 Rabat, Maroc
3Laboratoire de Biotechnologie, Valorisation et Protection des Agroressources, Faculté des Sciences et Techniques Guéliz, B.P. 618, 40 000 Marrakech, Maroc
4UR, Amélioration génétique des Plantes, Institut national de la Recherche agronomique F- 40 000 Marrakech, Maroc
5Institut national de la Recherche agronomique, Amélioration des plantes et Conservation des ressources phytogénétiques CRRA, BP 578, Meknès, Maroc
*Corresponding Author E-mail: douiraallal@hotmail.com
ABSTRACT
Haouzia and Dahbia Olive plants were inoculated with Phytophthora palmivora and Verticillium dahliae
with two inoculation techniques. These two species were respectively isolated from Souk El Arbaa and
Meknes. Both of V. dahliae and P. palmivora provoke defoliation and decline symptoms with a difference
on the period of incubation. Using Technique 1 of inoculation (plants were sprayed with the inoculum
suspension), the aerial fresh weight of the non inoculated Dahbia olive plants was 400 g and it was
respectively 178 and 155 g for the olive plants inoculated with P. palmivora and V. dahliae and 87 and 73
leaves were count on the olive plant inoculated respectively with P. palmivora and V. dahliae relative to
the controls (480 leaves). Using Technique 2 (roots of the plants were dipped in the prepared inoculum).
The aerial and root fresh weight of Haouzia olive plants inoculated with P. palmivora, V. dahliae were
respectively (382-124 g) and (462-305 g) compared to the control (654-904 g). V. dahliae had a negative
effect on the height of the inoculated Dahbia and Haouzia olive plants; height of Haouzia olive plants
were affected (85 cm) compared to control (111 cm). P. palmivora was highly isolated from the stems of
Dahbia (95 %) and Haouzia (83 %) olive plants and it was weakly isolated from the leaves petiole of the
Dahbia (33.33%) and Haouzia (22 %) olive. V. dahliae was only isolated from the roots of the inoculated
Dahbia (18%) and Haouzia (22.22 %) olive plants. The importance of these two pathogens of the olive
tree has been discussed in this work.
Keywords: Verticillium dahlia, Phytophthora palmivora, Wilt, Olive tree.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article:
Int. J. Pure App. Biosci. 2 (2): 28-38 (2014)
