-
No. 772, Basant Vihar, Kota
Rajasthan-324009 India
-
Call Us On
+91 9784677044
-
Mail Us @
editor@ijpab.com
Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2013 , Volume 1, Issue 1
Page No. : 20-27
Article doi: : http://dx.doi.org/10.18782
Clonal micropropagation and callus induction of Terminalia bellerica- An endangered plant
Jaya Dhaker1*, Suresh Singh1, Divyarajsinh A. Zala2, Humaira Naaz1, Priyanka Gehlot3
1Plant Tissue Culture Division, Vital Biotech Research Institute, Kota
2Amity University Jaipur
3 Lachoo Memorial College of Science & Technology, Jodhpur
*Corresponding Author Email: jaya_dhaker2008@yahoo.com
ABSTRACT
An in vitro micropropagation system has been developed for Terminalia bellerica Roxb., an important
Indian medicinal plant. Nodal segments obtained from 15-d-old aseptically grown seedlings were used as
explants. MS medium containing 2.0 mg/l BAP was found most suitable for culture initiation. Although
shoot multiplication was achieved on MS medium containing BAP and Kn, the maximum number of
shoots was obtained with 3.5 mg/l BAP+ 0.5 mg/l Kn. Best rooting response was observed on medium
containing quarter strength MS salts, 0.8% agar and 1.0 mg/l IBA. Plantlets were hardened initially in
culture room conditions and then transferred to misthouse. Maximum callus induction response was
observed on MS medium supplemented with 0.25 mg/l 2,4-D+ 0.3 mg/l Kn within 4 weeks from leaf
petiole.
Keywords: Callus, Bahera, micropropagation, shoot multiplication, Medicinal plant.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article:
Int. J. Pure App. Biosci. 1 (1): 20-27 (2013)
