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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2014 , Volume 2, Issue 1
Page No. : 248-257
Article doi: : http://dx.doi.org/10.18782
In vitro micropropagation of biofuel feedstock pongam [Pongamia pinnata (L.) Pierre] through cotyledonary node culture and assessment of clonal uniformity by RAPD markers
Gargibala Satapathy, Manikkannan Thirunavoukkarasu*, Priya Ranjan Behera and Prasanna Kumar Panda
Bioresources Engineering Department, CSIR-Institute of Minerals & Materials Technology, Bhubaneswar 751 013, Orissa, INDIA
*Corresponding Author E-mail: mtarasu@yahoo.com
ABSTRACT
Pongamia pinnata has recently been recognized as a viable source of seed-oil for production of biofuel to
meet the demand of the burgeoning biofuel industry. Although this tree is propagated by seeds,
germination and plant vigour decreases following storage of seeds for 3 months or more. Plants
propagated through stem cuttings are not deep rooted, hence not a preferred method of cloning this
valuable species. Hence, the aim of the present study was to develop an efficient and reproducible mass
propagation protocol for raising clonal garden of P. pinnata through in vitro cotyledonary node culture
and assessing its trueness to parent plant.
Keywords: cotyledonary nodes, genetic fidelity, micropropagation, Pongamia pinnata, thidiazuran.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article:
Int. J. Pure App. Biosci. 2 (1): 248-257 (2014)
