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The experiment was conducted during Rabi season in 2015-16 at Research Area of Agonomy, Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana, India which is situated at 29°10' N latitude and 75° 46' E longitude which is 215.2 metre above mean sea level to notice the effect of fertilizer levels and different combinations of biofertilizers on N content & uptake. Various RDF levels (50%, 75% and 100%) and different combinations of biofertilizers (uninoculated, Azotobacter, Azospirillum, PSB, biomix, Azotobacter + PSB and Azospirillum +PSB) were used as the treatments. The data recorded indicate that various fertilizer levels significantly influenced the N content and its uptake in grain and N uptake in straw. Increasing levels of fertilizer resulted in significant enhancement in N content and its uptake in grain and also N uptake in straw of barley. Among the seed inoculations with different combinations of biofertilizers, least value for N content in grain was obtained from uninoculated treatment 1.70% in the year 2015-16. Seed inoculation with Biomix recorded highest N uptake in grain as well as straw.

 Keywords: Fertilizer levels, Biofertilizers, Biomix, Nitrogen content and N uptake.