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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2019 , Volume 7, Issue 5
Page No. : 476-481
Article doi: : http://dx.doi.org/10.18782/2320-7051.7560
Isolation and Medium Optimization of Bacterial Lipase from Oil Mill
Krishna Patel* and Samir Parikh
Sir P.T. Science College, Modasa, Gujarat
*Corresponding Author E-mail: krishnapatel9330@gmail.com
Received: 10.06.2019 | Revised: 17.07.2019 | Accepted: 24.07.2019
ABSTRACT
Lipase (glycerol ester hydrolases EC 3,1,1,3) is a important biocatalyst because of their demand in various industrial application. In this study lipolytic bacteria isolate KK12 Bacillus spp. was isolate from oil contaminated sample by using tributyrine agar medium. Isolate KK12 bacillus spp gave the highest zone of hydrolysis with 25mm. Lipase production was improved by optimized for different oils, carbon sources, nitrogen sources, substrate, pH, temperature, metal ions. The highest lipase activity was 14U/l at 370C at pH 7.6. In this study isolate KK12 Bacillus spp giving best activity with peptone was 9.2U/l. Lipase activity was high in olive oil about 9.8U/l at 370C after 48hours incubation than other substrate. Other different parameters like pH, temp., metal ions also study in this research same all of these.
Keywords: Lipase, Optimization, Bacterial isolates, Lipase activity, Oil contaminated soil
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782/2320-7051.7560
Cite this article:
Patel, K., & Parikh, S. (2019). Isolation and Medium Optimization of Bacterial Lipase from Oil Mill, Ind. J. Pure App. Biosci. 7(5), 476-481. doi: http://dx.doi.org/10.18782/2320-7051.7560
