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RNA sequencing or next generation sequencing (NGS) has emerged as a revolutionary tool in genetics, genomics, and epigenomics. Having high reproducibility and accuracy and cost effectiveness compared to other sequencing technologies, NSG has enabled genome wide investigations of various phenomena including allele specific expression, novel alternative splicing, single nucleotide polymorphism, copy number variants and novel transcripts. It also holds promise in discovering de novo transcription/splice junctions and small RNAs with high specificity. While RNA-Seq is a relatively new method, it has already provided unprecedented insights into the transcriptional complexities of a variety of organisms, including yeast, mice, Arabidopsis, and humans.

Key words: RNA-seqencing, Transcriptome, cDNA libraries, Illumina technology.