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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2021, Volume : 9, Issue : 2
First page : (66) Last page : (76)
Article doi: : http://dx.doi.org/10.18782/2582-2845.7658

Analysis of Genetic Diversity of Neem Using RAPD Markers

Prithviraj Parvaiah Bhandare^1*, Tathagat Eknathrao Waghmare² and Gajanan Ramchandra Naik^3
1Dairy Production Section, ICAR-National Dairy Research Institute, Adugodi, Bengaluru, Karnataka, India
²Department of Microbiology & Biotechnology, Faculty of Food Sciences,
Szent Istvan University, Budapest, Hungary
³Central University of Karnataka, India
*Corresponding Author E-mail: prithvi.e87@gmail.com
Received: 5.02.2021 | Revised: 16.03.2021 | Accepted: 24.03.2021 

 ABSTRACT

The present investigation was carried out during 2013-14. RAPD molecular markers were used to evaluate the genetic diversity in populations of Azadirachta indica A. Juss (neem) from different regions of north Karnataka. Out of the 05 random decamer primers used, 03 yielded polymorphic banding patterns. In total, 23 different DNA bands were reproducibly obtained, out of which 18 (78.26%) were polymorphic. The polymorphisms were scored and data was plotted as a binary matrix to be used for phylogenetic analysis using PyElph 1.4 and PAST software. Cluster analysis based on Jaccard’s similarity coefficient using UPGMA grouped all the 20 populations into two major groups. The principal component analysis observed on an average 37% of the total variation.

Keyword: Azadirachta indica, genetic variation, DNA, RAPD, PCR.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Bhandare, P. P., Waghmare, T. E., & Naik, G. R. (2021). Analysis of Genetic Diversity of Neem Using RAPD Markers, Ind. J. Pure App. Biosci. 9(2), 66-76. doi: http://dx.doi.org/10.18782/2582-2845.7658