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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2021, Volume : 9, Issue : 2
First page : (240) Last page : (253)
Article doi: : http://dx.doi.org/10.18782/2582-2845.8214

Aptamer Selection: Choosing the Appropriate SELEX

Shilpi Malhotra¹, Satvinder Singh², Rajni Mala¹ and Seema Sood^1*
1Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India
²Dr. D. P. Rastogi Central Research Institute (Homeopathy), Noida, U.P., India
*Corresponding Author E-mail: seemalsood@rediffmail.com
Received: 13.12.2020 | Revised: 22.01.2021 | Accepted: 4.02.2021 

 ABSTRACT

Aptamers are oligonucleotides that have an innate ability to bind to various target molecules such as small molecules, toxins, oligonucleotides, proteins and even whole cells with high affinity and specificity. They emerged as strong rivals to the most commonly used biomolecules in the past, antibodies. The aptamer selection process involves evolution of ligands by iterative affinity assays termed as SELEX (Systematic Evolution of Ligands by Exponential Enrichment). This is a tedious task and efforts are being directed to simplify the process and at the same time reduce the turnaround time. Several variants have been designed to overcome the limitations of conventional SELEX process. This review provides sufficient information to a naive researcher for choosing and designing appropriate strategy for selection of aptamers against target of interest.

Keywords: Aptamers, SELEX, Target, Affinity.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Malhotra, S., Singh, S., Mala, R., & Sood, S. (2021). Aptamer Selection: Choosing the Appropriate SELEX, Ind. J. Pure App. Biosci. 9(2), 240-253. doi: http://dx.doi.org/10.18782/2582-2845.8214