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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2021, Volume : 9, Issue : 2
First page : (7) Last page : (18)
Article doi: : http://dx.doi.org/10.18782/2582-2845.8605

Molecular Characterization of Papaya Ring Spot Virus Isolates From Different Parts of Tamil Nadu

Nirmal Kumar Ramasamy^1*, Shobana V. G.², Kannan R.³, Muthukumaran P.⁴ and Angappan K.^5
1*Assistant Professor, Department of Biotechnology,
Sri Shakthi Institute of Engineering and Technology, Coimbatore, Tamil Nadu 641062, India
²Visiting Scientist, Department of Pearl Millet,
International Crops Research Institute for the Semi-Arid Tropics, Telengana, India
³Professor and Head, Department of Petrochemical Engineering,
RVS college of  Engineering  and Technology, Coimbatore, Tamil Nadu 641402, India
⁴Faculty in Science, Institute of Virtual & Distance Learning (IVDL),
DMI St. Eugene University, Chibombo, Zambia
⁵Professor, Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore
*Corresponding Author E-mail: nirmalbiotech50@gmail.com
Received: 17.01.2021 | Revised: 23.02.2021 | Accepted: 2.03.2021 

 ABSTRACT

An attempt has been made to characterize different PRSV isolates which infects papaya plants were collected from different parts of Tamil Nadu. The isolates were of PRSV-P type strain and the Coat Protein gene (CP) varied in size from 840- 860 bp which encodes 280 - 284 amino acids. Sequence alignment results revealed that the seven sequences obtained from different regions shared a homology of about 90% -95% with each other and homology of about 96% with the already reported Indian isolate and 88-90% with the other country isolates collected from NCBI. KE repeats were observed in the N terminus of the CP coding region from the different isolates found out. Phylogenetic analysis revealed that the isolates identified here placed on the same group and reference isolates were grouped in a different progeny. Our study further helped us to identify conserved regions among the seven isolates and we also constructed an RNAi silencing vector targeting Coat protein gene to challenge the Papaya Ring Spot Virus (PRSV) for our future study.

Keywords: Coat protein gene, Papaya Ring Spot Virus (PRSV), FAO

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Ramasamy, N. K., Shobana, V. G., Kannan, R., Muthukumaran, P., & Angappan, K. (2021). Molecular Characterization of Papaya Ring Spot Virus Isolates From Different Parts of Tamil Nadu, Ind. J. Pure App. Biosci. 9(2), 7-18. doi: http://dx.doi.org/10.18782/2582-2845.8605