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Ceropegia bulbosa Roxb. (family–Asclepiadaceae), is a slender, small, perennial, climber or twining tuberous herb with very wide distribution. Ceropegia is a genus of climbers, herbs, rarely sub shrubs and consists of about 200 species distributed in the tropical and subtropical Asia, Africa, Australia, Malaysia and Canary and Pacific islands (Anonymous, 1992; & Bruyns, 2003). In India it is found in Andhra Pradesh, Punjab, Rajasthan, Tamil Nadu and Uttar Pradesh. It is popularly known as Hedulo, Khedula, Tedula in Rajasthan and reported to occur sparsely in Sikar, Udaipur, Jalore, Kota districts. It has variable, elliptic-oblong to, acute or, base rounded leaves. The flowering and fruiting time of Ceropegia bulbosa is reported between July to October (Bhandari, 1990). Flowers are hermaphrodite, green, inpedunculate cymes, umbellate cymes, narrow and slightly funnel shaped. Follicles are cylindric, tapering towards apex. Seeds are flattened and ovate oblong. The tuber is spherical or oblong, turnip like with roots at its base.

As many as 48 spp. of Ceropegia are found in India however most of these are threatened owing to destructive collection or habitat degradation (Nayar & Sastry, 1987; Walter & Gillet, 1998; & Dhir & Shekhawat, 2014). They are therefore scarcely available in wild. Ceropegia bulbosa iscommonly distributed throughout the country (Botanical Survey of India, 2002).
            Ceropegia species are economically important due to their starchy edible tubers. The tuberous roots of Ceropegia bulbosa species are edible and also have medicinal properties (Arora & Meena, 2018a, 2018b). Tubers are a rich source of carbohydrates and eaten either raw or cooked (Nikam, 2007). The tubers are also used as a thirst quencher. Leaves are also edible and possess tonic and digestive properties. The root tubers are known to contain an alkaloid `Ceropegin’ (Nadkarni, 1976). Bitterness of the tubers can be removed by boiling (Mabberley, 1987). The alkaloid has medicinal value and is used to cure cold, sneezing and eye diseases. C. bulbosa seeds are brayed to a paste and dropped into the ears to cure deafness. Decoction of the tubers is used to remove urinary bladder stones (Swarnkar & Katewa, 2008; & Khan & Pradhan, 2012). The whole plant extract is used as an antidote for snake bite (Anonymous, 1980).
            Indigenous people use various wild plants for their food, medicines, and other economic products. Many of these wild plants have been documented for their utilization as future foods and medicines based on these people's information. However, information about the nutrient components of many wild edible plants has yet to be scientifically tested. There are only very few reports evaluating nutritional composition of Cerpegia bulbosa. Complete analysis of is lacking. Therefore, this study evaluated its nutrient components with an aim to explore its potential as an alternative food. This information may serve as a basis for conservation for its increased consumption and utilization.

MATERIALS AND METHODS

Collection & processing of material
Reconaissance survey was carried out in three different Districts of Rajasthan viz. Beda (Pali), Udaipur and Jaswantpura (Jalore) for collection of Ceropegia tubers for three consecutive years 2012-2014. Sites were selected by gathering information from local people, Forest Department and Universities. Collected tubers were washed with water to remove dirt and foreign materials and then cut into thin slices and dried in shade. Finally these were ground and stored in neatly labelled air tight bags.
Nutritional Analysis
All the chemicals used in the study were of analytical grade and procured from Merck. Standards were procured from SIGMA. All the values are mean of three readings.
Proximate analysis
Moisture, fat, crude protein, total sugar (TSS), ash and dietary fibre (TDF), were determined using standard methods (AOAC, 1984). Moisture was determined by oven drying method. Ash content was determined by incineration of the sample in a muffle furnace at 600ºC for 6 h. Total Dietary fibre was determined by assay kit (SIGMA). It is a combination of enzymatic and gravimetric methods. Samples were gelatinized with heat stable α-amylase and then enzymatically digested with protease and amylo glucosidase to remove the protein and starch present in the sample. Ethanol is added to precipitate the soluble dietary fibre. Total sugar content was estimated by phenol-sulphuric acid method. Total nitrogen was estimated by the Kjeldahl method. Crude protein was calculated by multiplying the value by 6.25. Fat was determined by petroleum ether extraction in a Soxhlet apparatus.
Vitamin C was determined by Indophenol Method (AOAC, 1984).
Mineral Analysis
Mineral elements (Cu, Zn, Fe, Mn, Mg) were determined by wet digestion methods using Atomic Absorption Spectrophotometer (AAS, Perkin Elmer). An acid digestion procedure was used for sample preparation. A weighed amount of plant material were placed in a digestion tube (Kjeldahl flasks) and a mixture of HNO3 and 70% HClO4 was added to each sample. Mixture was heated slowly at a low temperature. After digestion, the samples were diluted to appropriate volume and the concentration of the elements was determined. Standards for each element were prepared by suitable dilution of the stock standard solutions. K, Ca & Na were estimated by flame photometer (Systronics).

RESULTS AND DISCUSSION

Vegetative Characteristics