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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2021, Volume : 9, Issue : 1
First page : (457) Last page : (462)
Article doi: : http://dx.doi.org/10.18782/2582-2845.8551
Study the Fresh Seed Dormancy in Sesame (Sesamum indicum L.)
Sondarva Jyoti*, Patel J. B. and C. A. Babariya
Department of Seed Science and Technology, College of Agriculture, Junagadh Agriculture University
Junagadh – 362001, Gujarat, India
*Corresponding Author E-mail: sondarvajyoti6568@gmail.com
Received: 18.12.2020 | Revised: 24.01.2021 | Accepted: 1.02.2021
ABSTRACT
The sesame seed of five varieties viz., GT 2, GT 3, TKG 22, Pragati and GT 10 were produced in kharif season and of three varieties viz., GT 2, GT 3 and GT 5 were produced in summer season at two locations of the Junagadh Agricultural University, Junagadh namely Agricultural Research Station, JAU, Amreli and Sagdiwidi Farm, JAU, Junagadh. The seed of five varieties produced at both the locations were brought immediately to the Department of Seed Science and Technology, College of Agriculture, JAU, Junaagdh and the observations were recorded on germination percentage immediately followed by 10 days interval along with the old seeds supplied by Agricultural Research Station, JAU, Amreli, of all these five varieties. From the results, it was concluded that freshly harvested seeds of white seeded sesame varieties GT-2, GT-3, TKG 22, Pragati and GT-5 produced in the previous season could not be utilized for sowing, as seed dormancy was found in these varieties and it was released after 115, 115, 95, 105 and 105 days after harvesting, respectively. However, black seeded variety GT 10 could be utilized for sowing in the next season, as it released the dormancy after at 35 days after harvesting (DAH).
Keywords: Kharif, Germination, Genetics, Varieties
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Jyoti, S., Patel, J. B., & Babariya, C. A. (2021). Study the fresh seed dormancy in sesame (Sesamum indicum L.), Ind. J. Pure App. Biosci. 9(1), 457-462. doi: http://dx.doi.org/10.18782/2582-2845.8551
INTRODUCTION
In sesame, germination problem observed when summer produce is utilized for kharif sowing; one of the reasons would be the existence of dormancy. Some reports are available on seed dormancy in sesame as reported by Ashri and Palevitch (1979) in Mexican cultivar Cola de Burrego and Tanesaka et al. (2012) in S. mulayanum and F1 hybrid of S. mulayanum and S. indicum. No such studies of Indian sesame varieties in general and Gujarat varieties in particular have been carried out. Therefore, whether dormancy is present in Gujarat sesame varieties or not, a preliminary study was carried out by Department of Genetics and Plant Breeding, JAU, Junagadh. The study was carried out up to 30 days after harvesting and the results showed that dormancy in sesame seed is there.
Based on this preliminary observation and the issue of summer sesame produce to use it for kharif sowing, the experiment is framed out to know the extent of fresh seed dormancy present in sesame both in kharif and summer seed production.
MATERIALS AND METHODS
The experiment material comprises of sesame seed of five varieties viz., GT 2, GT 3, TKG 22, Pragati and GT 10 were produced in kharif season (kharif 2016, 2017 & 2018) and of three varieties viz., GT 2, GT 3 and GT 5 were produced in summer season (summer 2017, 2018 & 2019) at two locations of the Junagadh Agricultural University, Junagadh namely Agricultural Research Station, JAU, Amreli and Sagdiwidi Farm, JAU, Junagadh to know the extent of fresh seed dormancy in sesame both in kharif and summer seed production. The seed of five varieties produced at both the locations were brought immediately to the Department of Seed Science and Technology, College of Agriculture, JAU, Junaagdh and the observations were recorded on germination percentage immediately (5 days after harvesting) followed by 10 days interval until the germination percentage reaches above 80 per cent, which is the Indian Minimum Seed Certification Standard for sesame. The old seeds supplied by Agricultural Research Station, JAU, Amreli, of all these five varieties were used as check. The statistical analysis carried out as per Factorial complete randomized block design.
RESULT AND DISCUSSION
The seed of five varieties viz., GT 2, GT 3, TKG 22, Pragati and GT 10 were produced at both the locations (Agricultural Research Station, JAU, Amreli and Sagdiwidi Farm, JAU, Junagadh) during kharif 2016, 2017 and 2018. However, the seed production at Sagdividi Farm, JAU, Junagadh was failed due to high rainfall at harvesting stage in kharif 2016, while the seeds of three varieties viz., GT 2, GT 3, and GT 5 were produced during summer 2017, 2018 and 2019 at both the above locations.
The results of Amreli location of kharif 2016 revealed the presence of seed dormancy in sesame (Table 1). The old seeds are germinated, but amongst the fresh seeds of the five varieties, GT 10 was reached to the optimum germination (above 80 %) after 25 DAH; TKG 22 and Pragati were reached to optimum germination after 105 DAH; while in remaining two variety. GT 2 was reached to optimum germination at 115 DAH and GT 3 was reached to optimum germination at 125 DAH.
The results of seed produced during kharif 2017 at both the locations (Table 1) revealed the presence of seed dormancy in sesame. The old seeds are germinated as per the seed standards right from first date of observation, while the fresh seeds showed seed dormancy. Of the five varieties, GT 10 was reached to the optimum germination (above 80 %) after 25 DAH in Amreli location and 35 DAH at Junagadh location. Variety TKG 22 was reached to optimum germination after 75 DAH and 105 DAH at Amreli and Junagadh, respectively. Pragati was reached to optimum germination at 115 DAH at Amreli location and 125 DAH at Junagadh location. GT 2 reached to optimum germination at 105 DAH at Amreli location and 125 DAH at Junagadh location, while GT 3 reached to optimum germination at 85 DAH at Amreli location and 125 DAH at Junagadh location.
The results of seed produced during kharif 2018 at both the locations (Table 1) revealed the presence of seed dormancy in sesame. Of the five varieties, GT 10 reached to the optimum germination (above 80 %) after 55 DAH in Amreli and 45 DAH at Junagadh location. TKG 22 reached to optimum germination after 85 DAH and 95 DAHat Amreli and Junagadh, respectively. Pragati reached to optimum germination at 95 DAH at both the locations. GT 3 reached to optimum germination at 105 DAH at both the locations, while GT 2 was reached to optimum germination at 115 DAH at both the locations.
The results of seed produced during summer 2017 at both the locations (Table 1) revealed the presence of seed dormancy in sesame. The old seeds are germinated as per the seed standards right from first date of observation, while the fresh seeds showed seed dormancy. Of the three varieties, GT 5 was reached to the optimum germination (above 80 %) after 65 days of harvest at Amreli, while it reached to the optimum germination (above 80 %) after 125 days of harvest at Junagadh. Varieties GT 2 and GT 3 reached to the germination above 80 percent after 145 days of harvest at both the locations.
The results of seed produced during summer 2018 at both the locations (Table 1) revealed the presence of seed dormancy in sesame. Of the three varieties, GT 5 was reached to the optimum germination (above 80 %) after 75 DAH at Amreli location and 95 DAH at Junagadh location, while GT 3 reached to the germination above 80 per cent after 125 DAH at both the location, whereas GT 2 reached to the optimum germination after 125 DAH at Amreli location and 115 DAH at Junagadh location.
The results of seed produced during summer 2019 at both the locations (Table 1) revealed the presence of seed dormancy in sesame. Of the three varieties, GT 5 was reached to the optimum germination (above 80 %) after 85 DAH at Amreli and 95 DAH at Junagadh location, while GT 2 and GT 3 both reached to the germination above 80 percent after 115 DAH at Amreli and 105 DAH at Junagadh location.
From the above results, it can be concluded that seed dormancy is present in sesame. Among the different varieties tested, on an average, black seeded variety GT 10 released the dormancy (as per the IMSCS, > 80 %) at 35-40 DAH, while all the white seeded varieties tested released the dormancy after 100-115 DAH.
Table 1: Germination (%) of fresh harvested seeds of kharif season from ARS, Amreli and Sagdividi Farm, Junagadh
Variety / Period of observation |
Germination (%) |
||||||||||||
5 |
15 |
25 DAH |
35 DAH |
45 DAH |
55 DAH |
65 DAH |
75 DAH |
85 DAH |
95 DAH |
105 DAH |
115 DAH |
125 DAH |
|
Fresh seeds ( Kharif 2016) produced at Amreli |
|||||||||||||
GT-2 |
0.33 |
1.33 |
2.67 |
2.67 |
2.67 |
3.00 |
4.67 |
14.33 |
17.67 |
18.67 |
66.67 |
82.67 |
92.67 |
GT-3 |
0.00 |
0.00 |
1.33 |
1.67 |
4.67 |
6.33 |
8.67 |
9.33 |
9.67 |
11.33 |
25.00 |
75.33 |
85.67 |
TKG-22 |
4.67 |
5.67 |
11.33 |
14.00 |
17.00 |
33.67 |
48.33 |
70.67 |
71.00 |
72.00 |
80.45 |
90.00 |
92.00 |
Pragati |
0.67 |
1.00 |
6.00 |
6.33 |
11.67 |
16.33 |
30.67 |
58.67 |
60.67 |
62.33 |
85.33 |
85.67 |
87.33 |
GT-10 |
53.33 |
60.00 |
88.33 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
*First year data of variety GT-10 was taken up to 25 DAH when germination reached 88.33%, so analysis was not possible for this year |
|||||||||||||
Fresh seeds ( Kharif 2017) produced at Amreli |
|||||||||||||
GT-2 |
7.00 |
12.00 |
13.33 |
17.00 |
22.67 |
25.00 |
25.67 |
27.67 |
68.33 |
78.00 |
81.33 |
90.67 |
92.33 |
GT-3 |
3.00 |
5.33 |
7.00 |
8.67 |
14.67 |
20.33 |
25.00 |
62.00 |
84.33 |
88.67 |
87.67 |
97.33 |
98.33 |
TKG-22 |
22.67 |
24.33 |
25.67 |
26.67 |
50.67 |
54.00 |
55.67 |
83.33 |
87.67 |
90.33 |
93.33 |
97.33 |
99.00 |
Pragati |
10.00 |
14.00 |
16.33 |
17.00 |
32.00 |
33.00 |
37.00 |
52.33 |
62.67 |
64.67 |
77.67 |
84.67 |
99.00 |
GT-10 |
72.00 |
79.00 |
84.33 |
88.00 |
94.33 |
96.00 |
97.00 |
97.67 |
99.00 |
99.00 |
99.00 |
99.33 |
99.00 |
|
Variety (A) |
DAH (B) |
A X B |
|
|||||||||
SEM |
0.39 |
0.63 |
1.42 |
||||||||||
C.D. |
1.10 |
1.78 |
4.00 |
||||||||||
C.V.% |
4.26
|
Table 1: Contd....
Fresh seeds ( Kharif 2018) produced at Amreli |
|||||||||||||
GT-2 |
8.67 |
17.67 |
22.00 |
28.00 |
37.67 |
46.00 |
57.67 |
66.00 |
68.67 |
73.67 |
74.67 |
85.67 |
95.67 |
GT-3 |
11.33 |
20.33 |
26.67 |
38.00 |
46.00 |
55.00 |
63.00 |
70.00 |
72.33 |
78.00 |
83.33 |
90.33 |
97.67 |
TKG-22 |
44.33 |
44.67 |
46.67 |
51.33 |
51.67 |
61.33 |
68.00 |
77.00 |
86.33 |
93.33 |
97.33 |
96.33 |
96.67 |
Pragati |
50.00 |
46.33 |
50.00 |
53.67 |
55.33 |
65.33 |
71.00 |
74.67 |
76.33 |
86.67 |
95.67 |
96.33 |
94.67 |
GT-10
|
54.33 |
57.00 |
60.67 |
72.33 |
79.67 |
83.33 |
87.00 |
88.67 |
91.67 |
98.33 |
97.67 |
99.00 |
98.33 |
|
Variety (A) |
DAH (B) |
A X B |
|
|||||||||
SEM |
0.58 |
0.93 |
2.09 |
||||||||||
C.D. |
1.62 |
2.62 |
5.86 |
||||||||||
C.V.% |
5.44 |
||||||||||||
Fresh seeds ( Kharif 2017) produced at Sagdividi (Junagadh) |
|||||||||||||
GT-2 |
4.00 |
5.33 |
6.67 |
17.67 |
19.00 |
21.33 |
22.33 |
24.33 |
49.00 |
54.33 |
68.67 |
77.33 |
84.33 |
GT-3 |
6.67 |
8.00 |
9.33 |
17.00 |
18.67 |
21.33 |
24.33 |
26.33 |
47.33 |
56.00 |
69.67 |
70.33 |
86.33 |
TKG-22 |
9.33 |
11.33 |
12.67 |
21.67 |
29.33 |
30.00 |
32.33 |
58.00 |
64.67 |
76.00 |
80.33 |
87.00 |
90.33 |
Pragati |
3.33 |
6.00 |
9.67 |
17.67 |
19.00 |
32.00 |
33.67 |
43.33 |
60.00 |
66.00 |
72.67 |
79.00 |
86.67 |
GT-10 |
25.67 |
27.66 |
45.00 |
93.67 |
94.00 |
94.67 |
96.67 |
98.00 |
99.00 |
99.00 |
99.33 |
99.00 |
99.67 |
|
Variety (A) |
DAH (B) |
A X B |
|
|||||||||
SEM |
0.57 |
0.92 |
2.05 |
||||||||||
C.D. |
1.59 |
2.57 |
5.76 |
||||||||||
C.V.% |
7.43 |
Table 1: Contd....
Fresh seeds ( Kharif 2018) produced at Sagdividi (Junagadh) |
|||||||||||||
GT-2 |
7.33 |
15.67 |
21.00 |
35.67 |
38.33 |
39.00 |
49.67 |
60.00 |
68.00 |
75.00 |
77.67 |
84.67 |
93.33 |
GT-3 |
10.67 |
21.67 |
27.67 |
34.33 |
48.00 |
45.00 |
51.33 |
63.33 |
70.33 |
75.33 |
84.67 |
94.33 |
97.67 |
TKG-22 |
29.33 |
37.67 |
42.33 |
51.00 |
54.33 |
62.67 |
61.33 |
69.00 |
77.67 |
86.33 |
89.00 |
92.67 |
95.00 |
Pragati |
37.67 |
43.33 |
52.67 |
56.67 |
57.67 |
57.00 |
66.67 |
70.33 |
77.00 |
83.67 |
96.00 |
93.67 |
96.33 |
GT-10
|
49.00 |
65.67 |
75.00 |
78.00 |
82.67 |
87.00 |
88.67 |
93.00 |
95.00 |
97.67 |
97.33 |
98.33 |
98.67 |
|
Variety (A) |
DAH (B) |
A X B |
|
|||||||||
SEM |
0.57 |
0.92 |
2.06 |
||||||||||
C.D. |
1.60 |
2.58 |
5.77 |
||||||||||
C.V.% |
5.49 |
||||||||||||
Mean germination percentage over the years and locations during Kharif season |
|||||||||||||
GT-2 |
5.47 |
10.40 |
13.13 |
20.20 |
24.07 |
26.87 |
32.00 |
38.47 |
54.33 |
59.93 |
73.80 |
84.20 |
91.67 |
GT-3 |
6.33 |
11.07 |
14.40 |
19.93 |
26.40 |
29.60 |
34.47 |
46.20 |
56.80 |
61.87 |
70.07 |
85.53 |
93.13 |
TKG-22 |
22.07 |
24.73 |
27.73 |
32.93 |
40.60 |
48.33 |
53.13 |
71.60 |
77.47 |
83.60 |
88.09 |
92.67 |
94.60 |
Pragati |
20.33 |
22.13 |
26.93 |
30.27 |
35.13 |
40.73 |
47.80 |
59.87 |
67.33 |
72.67 |
85.47 |
87.87 |
92.80 |
GT-10
|
50.87 |
57.87 |
70.67 |
83.00 |
87.67 |
90.25 |
92.34 |
94.34 |
96.17 |
98.50 |
98.33 |
98.92 |
98.92 |
Table 2: Germination (%) of fresh harvested seeds of summer season from ARS, Amreli and Sagdividi (Junagadh)
Variety / Period of observation |
Germination (%) Fresh seeds produced at ARS, Amreli and Saddividi (Junagadh) |
||||||||||||||
5 |
15 DAH |
25 DAH |
35 DAH |
45 DAH |
55 DAH |
65 DAH |
75 DAH |
85 DAH |
95 DAH |
105 |
115 |
125 |
135 |
145 |
|
Fresh seeds (Summer 2017) produced at ARS, Amreli |
|
|
|
|
|
||||||||||
GT-2 |
9.33 |
13.67 |
16.67 |
24.33 |
25.67 |
30.67 |
43.00 |
49.00 |
53.67 |
62.67 |
67.33 |
69.67 |
71.33 |
73.67 |
84.33 |
GT-3 |
17.67 |
24.67 |
31.33 |
38.00 |
41.00 |
46.00 |
52.00 |
55.67 |
55.33 |
65.00 |
67.33 |
71.33 |
74.67 |
77.33 |
84.33 |
GT-5 |
19.67 |
21.00 |
26.00 |
45.67 |
72.33 |
79.67 |
80.33 |
81.00 |
81.33 |
84.33 |
84.67 |
86.67 |
86.33 |
87.33 |
91.00 |
|
Variety (A) |
DAH (B) |
A X B |
|
|||||||||||
SEM |
0.61 |
1.36 |
2.35 |
||||||||||||
C.D. |
1.71 |
3.82 |
6.61 |
||||||||||||
C.V.% |
7.26 |
||||||||||||||
Fresh seeds (Summer 2018) produced at ARS, Amreli |
|||||||||||||||
GT-2 |
27.33 |
32.00 |
41.33 |
46.00 |
48.67 |
56 |
64.67 |
66.00 |
68.00 |
70.67 |
77.33 |
79.67 |
82.00 |
88.00 |
|
GT-3 |
17.67 |
20.00 |
31.33 |
38.67 |
43.33 |
50.67 |
54 |
62.33 |
66.67 |
75.00 |
79.00 |
82.67 |
87.67 |
91.33 |
|
GT-5 |
25.00 |
31.33 |
33.33 |
41.33 |
51.33 |
62.00 |
76.00 |
85.33 |
85.33 |
87.33 |
90.00 |
89.00 |
99.00 |
98.00 |
|
|
Variety (A) |
DAH (B) |
A X B |
|
|||||||||||
SEM |
0.52 |
1.13 |
1.95 |
||||||||||||
C.D. |
1.47 |
3.18 |
5.51 |
||||||||||||
C.V.% |
5.48 |
Table 2: Contd....
Fresh seeds (Summer 2019) produced at ARS, Amreli |
|||||||||||||||
GT-2 |
28.33 |
32.67 |
38.00 |
41.00 |
48.00 |
53.33 |
62.33 |
65.67 |
68.00 |
71.33 |
77.00 |
83.00 |
83.33 |
89.33 |
|
GT-3 |
16.33 |
21.00 |
28.33 |
34.67 |
39.33 |
49.00 |
51.33 |
60.00 |
66.67 |
75.00 |
79.00 |
84.00 |
87.33 |
95.00 |
|
GT-5 |
24.33 |
30.00 |
34.33 |
39.00 |
46.33 |
56.00 |
64.33 |
77.67 |
83.33 |
90.00 |
91.67 |
92.33 |
97.33 |
98.00 |
|
|
Variety (A) |
DAH (B) |
A X B |
|
|||||||||||
SEM |
0.52 |
1.13 |
1.95 |
||||||||||||
C.D. |
1.47 |
3.17 |
5.50 |
||||||||||||
C.V.% |
5.57 |
||||||||||||||
Fresh seeds (Summer 2017) produced at Sagdividi (Junagadh) |
|||||||||||||||
GT-2 |
0.00 |
0.33 |
6.00 |
9.00 |
10.00 |
10.67 |
12.67 |
17.67 |
19.00 |
23.67 |
34.33 |
62.67 |
73.33 |
74.33 |
85.67 |
GT-3 |
0.00 |
0.67 |
1.00 |
1.67 |
6.00 |
6.00 |
10.33 |
12.33 |
14.00 |
34.33 |
40.67 |
57.33 |
65.00 |
76.33 |
82.33 |
GT-5 |
7.33 |
16.00 |
27.00 |
35.67 |
43.33 |
45.67 |
46.33 |
46.66 |
47.33 |
52.00 |
54.67 |
68.67 |
81.00 |
84.67 |
93.00 |
|
Variety (A) |
DAH (B) |
A X B |
|
|||||||||||
SEM |
0.53 |
1.19 |
2.06 |
||||||||||||
C.D. |
1.49 |
3.34 |
5.80 |
||||||||||||
C.V.% |
10.07 |
||||||||||||||
Fresh seeds (Summer 2018) produced at Sagdividi (Junagadh) |
|||||||||||||||
GT-2 |
26.67 |
31.00 |
42.33 |
49.33 |
54.33 |
55.33 |
62.33 |
69.67 |
71.00 |
79.00 |
83.33 |
84.67 |
86.33 |
87.67 |
|
GT-3 |
13.67 |
20.67 |
27.00 |
35.67 |
40.33 |
45.67 |
51.67 |
60.67 |
67.00 |
71.33 |
79.33 |
83.67 |
84.00 |
87.00 |
|
GT-5 |
27.00 |
33.33 |
38.00 |
42.33 |
44.00 |
40.33 |
50.00 |
60.67 |
70.33 |
81.00 |
85.67 |
88.00 |
86.33 |
86.67 |
|
|
Variety (A) |
DAH (B) |
A X B |
|
|||||||||||
SEM |
0.57 |
1.24 |
2.15 |
||||||||||||
C.D. |
1.61 |
3.49 |
6.05 |
||||||||||||
C.V.% |
6.30 |
Table 2: Contd....
Fresh seeds (Summer 2019) produced at Sagdividi (Junagadh) |
|
|
|||||||||||||
GT-2 |
30.00 |
31.00 |
39.00 |
42.67 |
50.67 |
54.67 |
66.33 |
73.00 |
74.67 |
77.67 |
80.67 |
87.33 |
86.33 |
91.67 |
|
GT-3 |
17.00 |
20.00 |
25.33 |
35.33 |
40.00 |
44.67 |
50.33 |
58.00 |
71.67 |
78.67 |
82.67 |
86.33 |
87.00 |
96.33 |
|
GT-5 |
28.00 |
32.67 |
37.00 |
41.33 |
44.33 |
47.00 |
51.00 |
60.00 |
73.67 |
81.33 |
84.67 |
84.67 |
86.33 |
98.00 |
|
|
Variety (A) |
DAH (B) |
A X B |
|
|||||||||||
SEM |
0.51 |
1.10 |
1.90 |
||||||||||||
C.D. |
1.43 |
3.09 |
5.36 |
||||||||||||
C.V.% |
5.49 |
||||||||||||||
Mean germination percentage over the years and locations during summer season |
|||||||||||||||
GT-2 |
20.28 |
23.45 |
30.56 |
35.39 |
39.56 |
43.45 |
51.89 |
56.84 |
59.06 |
64.17 |
70.00 |
77.84 |
80.44 |
84.11 |
85.00 |
GT-3 |
13.72 |
17.84 |
24.05 |
30.67 |
35.00 |
40.34 |
44.94 |
51.50 |
56.89 |
66.56 |
71.33 |
77.56 |
80.95 |
87.22 |
83.33 |
GT-5 |
21.89 |
27.39 |
32.61 |
40.89 |
50.28 |
55.11 |
61.33 |
68.56 |
73.55 |
79.33 |
81.89 |
84.89 |
89.39 |
92.11 |
92.00 |
CONCLUSION
The experiment concluded that freshly harvested seeds of white seeded sesame varieties GT-2, GT-3, TKG 22, Pragati and GT-5 produced in the previous season could not be utilized for sowing, as seed dormancy was found in these varieties and it was released after 115, 115, 95, 105 and 105 days after harvesting, respectively. However, black seeded variety GT 10 could be utilized for sowing in the next season, as it released the dormancy after at 35 days after harvesting (DAH).
REFERENCES
Ashri, A., & Palevitch, D. (1979). Seed dormancy in sesame and the effect of gibberrelic acid. Experimental Agriculture, 15, 81-83.
Tanesaka, E., Umeda, E., Yamamoto, M., Masuda, K., Yamada, K., & Yoshida, M. (2012). Inheritance mode of seed dormancy in the hybrid progeny of sesame, S. indicum, and its wild relative, S. mulayanum Nair. Weed Biology Management, 12, 91-97.