INDIAN JOURNAL OF PURE & APPLIED BIOSCIENCES

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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2021, Volume : 9, Issue : 1
First page : (297) Last page : (302)
Article doi: : http://dx.doi.org/10.18782/2582-2845.8600

Studies on Genetic Diversity and Variability for Yield and Yield attributes in Garlic (Allium sativum L.) Under Dhampur Condition

Deepak Kumar1* and Vikas Kumar2
1Department of Horticulture, R.S.M (PG) College, Dhampur, Bijnor-246761, Uttar Pradesh
2Department of Agriculture, Krishi Bhavan, Rampur- 244901, Uttar Pradesh
*Corresponding Author E-mail: deepak1231996@gmail.com
Received: 4.01.2020 | Revised: 11.02.2020 | Accepted: 17.02.2021 

 ABSTRACT

The experiment was conducted at Horticulture Research Farm, Dhampur, Bijnor during rabi season of 2019-20. In the present investigation 21 genotypes of garlic (Allium sativum L.) were evaluated in RBD with the objective of estimating the genetic variability and determination of association among different attributes with each other and with bulb yield. Analysis of variance for design of experiment revealed that there is the weight of fresh bulbs had highly significant and positive correlation with plant equatorial diameter, weight of dry bulbs, leaf length, sulphur contain indicated that selection for these traits would be effective for the improvement of yield (q/ha). The maximum positive direct effect on yield (q/ha) was exerted by number of cloves/bulb, leaf width, polar diameter, plant height (cm), height of pseudo stem, total soluble solid, protein(%), plant pseudo stem diameter, width of leaf and number of leaves/plant. It is suggested that selection for these traits will directly increase yield (q/ha).

Keywords: Garlic, Variability, Correlation, RBD, Allium sativum.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Kumar, D., & Kumar, V. (2021). Studies on Genetic Diversity and Variability for Yield and Yield attributes in Garlic (Allium sativum L.) Under Dhampur Condition, Ind. J. Pure App. Biosci. 9(1), 297-302. http://dx.doi.org/10.18782/2582-2845.8600

INTRODUCTION

Garlic (Allium sativum L.) belongs to the family Alliaceae (Allen, 2009).  It is an apomyctic diploid species (2n=2x=16). The origin of garlic is thought to be in central Asia (India, Afghanistan, West China, Russia) and spread to other parts of the world through trade and colonization (Tindal, 1986). Garlic has been used in china and India for more than 500 years, and Egypt since 2000 B.C (Kamenetsky & Rabinowitch, 2001). Garlic is the most important Allium crops and ranks second next to onion in the world (Voigt, 2004).

The total area under garlic cultivation in India is (2.45 million hectare) and production is (3.65 million tonnes) with productivity of (18.72 million tonnes/hectare) (NHRDF, 2020). With respect to its production and economic value, garlic is one of the main Allium vegetable crops in the world and used as a seasoning in many food throughout the globe. Garlic has also medicinal value which is well recognized in the control and treatment of hypertension, worms, germ, bacterial and fungal diseases diabetes, cancer, ulcer, rheumatism etc.

(Kilgori et al., 2007(b) Samavatean et al., 2011, & Kamenetsky et al., 2006) Many people perceived and appreciated garlic for its many medicinal attributes (Rabinowitch & Currah, 2002).


MATERIALS AND METHODS

The field experiment was conducted at Horticulture Research Farm (HRF) at R.S.M (PG) College, Dhampur, Bijnor, Uttar Pradesh India. Geographically it is situated between 290 15’ 27.5328” latitude in the north and 78o 30’ 0.2196” longitudes in the eastern elevation of about 235 m above mean sea level. The experimental materials of garlic used in the present study were collected from different places of India. The experiment was laid out in Randomize Block Design (RBD). These 21 genotypes were evaluated and studied for their growth, yield and quality performance based on morphological and agronomical measurements. The following observations were recorded during the course of experimentation on following characters like-Plant height (cm), number of leaves/plant, length of leaf (cm), width of leaf (cm), plant pseudo stem height (cm), plant pseudo stem diameter (mm), equatorial diameter of bulbs (mm), polar diameter of bulbs (mm), weight of fresh bulbs (g), weight of dry bulbs (g), number of cloves/bulb, yield (q/ha), total soluble solids (%). protein (%), sulphur contain (%). These observations were recorded on five randomly selected plants of each row. Averages of data from the sampled plant of each treatment were used for statistical analysis in order to draw valid conclusion. The statistical parameters like- mean, ranges were calculated as per the standard methods of analysis.

RESULTS AND DISCUSSION

The analysis of variance for the design of experiment indicated that the mean squares due to genotypes were highly significant for most of the characters indicating a wide genetic variability among the genotypes in Table 1. The variation due to checks were also highly significant for are the characters like- plant height (58.91 cm), Plant pseudostem height (3.87 cm), number of leaves per plant (1.06), length of leaf (15.68 cm), width of leaf (6.72 mm), Plant pseudostem diameter (3.97 mm), Equatorial diameter of bulbs (25.71 mm), Polar diameter of  bulbs (20.07 mm),  number of cloves per bulb (22.96), weight of fresh bulbs  (152.72 g),  weight of dry bulbs (122.57 g), Yield quantal/ha (1573.66),  total soluble solids (9.25%), Sulphur contain (0.0005 %), Protein (0.30 %). Mean performance serves as an important criterion in eliminating the undesirable types in a selection progrmme.Tesega K and Tiwari (2016)10. The phenotypic  correlation coefficients were worked out to measure the association among the fiften characters under study. The estimates of these correlation coefficients are presented in table-2. Plant height showed highly positive and significant correlation at phenotypic level with weight of fresh bulbs (0.562), leaf length (0.459), weight of dry bulb (0455), Equatorial Diameter of bulbs (0.440) and polar diameter (0.313), whereas sulphur content (-246) showed negative and significant correlation with the trait.Plant pseudo-stem height showed positive and significant correlation with number of leaf (0.567), leaf width (0.319). Whereas negative and significant correlation with polar diameter (-0.484), total soluble solids (-0.273).Number of leaf showed positive and significant correlation with leaf width (0.708), polar diameter (0.541), weight of dry bulbs (0.478), pseudo-stem diameter (0.436), yield q/ha (0.427), weight of fresh bulbs (0.415), equatorial diameter of bulbs (0.364), and total soluble solids (0.247). Leaf length showed positive and significant correlation with weight of fresh bulbs (0.437), plant pseudo-stem diameter (0.433), weight of dry bulbs (0.377), leaf width (0.284) and equatorial diameter of bulbs (0.261).  Leaf width showed positive and significant correlation with plant pseudo-stem diameter (0.583), yield q/ha (0.494), total soluble solids (0.436), polar diameter (0.425), weight of dry bulbs (0.411), weight of fresh bulbs (0.397), and equatorial diameter (0.343). All other traits were not found to be significantly correlated with the leaf width. Plant pseudo-stem diameter showed positive and significant correlation with weight of fresh bulbs (0.673), weight of dry bulbs (0.659), yield q/ha (0594), equatorial diameter of bulbs (0.511), number of cloves/ bulbs (0.444), polar diameter (0.382), protein content (0.348), and total soluble solids (0.338). Equatorial diameter of bulbs showed positive and significant correlation with weight of dry bulbs (0.764), weight of fresh bulbs (0.755), polar diameter (0.370) and yield q/ha (0.323), whereas positive and non significant correlation with protein (0.175) and sulphur content (0.001) and total soluble solids (-0.81). Polar diameter showed positive and significant correlation with weight of dry bulbs (0.493), weight of fresh bulbs (0.462) and yield q/ha (0.258) whereas negative and significant correlation with sulphur content (-0.276). Number of cloves/plant showed positive and significant correlation with yield q/ha (0.362), sulphur content (0.338), weight of dry bulbs (0.328), weight of fresh bulbs (0.287), and protein (0.296).Weight of fresh bulbs showed positive and significant correlation with weight of dry bulbs (0.562), leaf length (0.437), yield q/ha (0.450) and protein (0.320) whereas positive and non significant correlation with total soluble solids (0.156) and sulphur content (-0.131) was recorded. Yield q/ha showed positive and significant correlation protein (0.428) highly significant and total soluble solids (0.300) significant. Total soluble solids showed positive and significant correlation protein (0.429) highly significant whereas positive and non significant correlation was found with sulphur contain (0.023). The genotypic correlation coefficients were worked out to measure the association among the fifteen characters under study. The estimates of these correlation coefficients are presented in table-3 Plant height showed positive and significant correlation with weight of fresh bulbs (0.663), Equatorial diameter (0.608), weight of dry bulbs (0.595), leaf length (0.504), sulphur contain (0.406) whereas negative and significant correlation with total soluble solids (-0.305) and protein content (-0.259). Plant pseudo-stem height showed positive and significant correlation with leaf width (0.922), number of leaf (0.889), protein content (0.481), total soluble solids (0.439), weight of dry bulbs (0.354), number of cloves/plant (0.347) and sulphur content (0.326) whereas genotype negative and significant correlation polar diameter (-0.708) highly significant and weight of fresh bulbs (-0.305). Plant pseudo-stem height showed positive and significant correlation with leaf width (1.031), polar diameter (0.757), weight of dry bulbs (0.564), yield q/ha (0.526), plant pseudo-stem diameter (0.512), weight of fresh bulbs (0.437), protein (0.362), equatorial diameter of bulbs (0.348) and total soluble solids (0.306). Leaf length showed positive and significant correlation with weight of fresh bulbs (0.756), weight of dry bulbs (0.744), plant pseudo-stem diameter (0.582), equatorial diameter bulbs (0.530) highly significant, yield q/ha (0.261) and polar diameter (0.258). Leaf width showed positive and significant correlation with plant pseudo-stem diameter (0.874) highly significant, weight of dry bulbs (0.846) highly significant, yield q/ha (0.832), polar diameter (0.771), weight of fresh bulbs (0.693), protein (0.585), equatorial diameter of bulbs (0.524), total soluble solids (0.463), number of cloves/plant (0.282). Plant pseudo-stem diameter showed positive and significant correlation with yield q/ha (0.833), weight of dry bulbs (0.825), weight of fresh bulbs (0.823), protein (0.694), equatorial pseudo-stem diameter (0.649), number of cloves/plant (0.576), number of cloves/plant (0.576), number of cloves/plant (0.576), number of cloves/plant (0.576), polar diameter (0.484).Equatorial diameter of bulbs showed positive and significant correlation with weight of dry bulbs (0.947), weight of fresh bulbs (0.931), yield q/ha (0.507) ), polar diameter (0.483) ), protein (0.409) ) and  total soluble solids (0.341) ). Polar diameter showed positive and significant correlation with weight of dry bulbs (0.651), weight of fresh bulbs (0.585) ), protein (0.530) ), yield q/ha (0.467) ) and sulphur contain (0.436) ). Number of cloves/bulbs showed positive and significant correlation with protein (0.716), yield q/ha (0.606), sulphur contain (0.490), weight of dry bulbs (0.470), and weight of fresh bulbs (0.368). Weight of fresh bulbs showed positive and significant correlation with weight of dry bulbs (0.961), yield q/ha (0.628), protein (0.557). Weight of dry bulbs showed positive and significant correlation with yield q/ha (0.694), and protein (0.559). Yield q/ha showed positive and significant correlation with protein (0.759) and total soluble solids (o.534). Total soluble solids showed positive and significant correlation with protein (0.819); and Sulphur contains showed positive and significant correlation with protein (0.399). In general the estimates of genotypic correlation coefficients between different characters showed close parallelism in direction with their corresponding phenotypic correlation coefficients presented in Table 2 and Table 3. Similar results were reported by, Tiwari et al. (2014), Tsegas & kasshum (2011), Yadav & Singh (2007), Yadav & Singh, (2012), Pense et al. (2014) and Peryin et al. (2014).

Table 1: Mean sum squares genotypes


Source of variation

d.f.

Plant height(cm)

Plant pseudo stem height(cm)

Number of leaves/plant

Leaf length(cm)

Leaf Width (mm)

Plant pseudo stem diameter(mm)

Equatorial diameter of bulbs(mm)

Polar diameter(mm)

Replication

2

2.22

1.14

0.32

8.20

0.02

1.57

5.70

0.74

Treatment

20

58.91**

3.87**

1.06**

15.68**

6.72**

3.97**

25.71**

20.07**

Error

40

6.28

0.87

0.15

4.34

2.08

0.46

3.81

2.20

Source of variation

d.f.

Number of cloves/bulb

Weight of fresh bulbs(g)

Weight of dry bulbs(g)

Yield(q/ha)

Total soluble solids %

Sulphur content %

Protein %

Replication

2

4.06

35.27

42.67

232.21

4.55

0.0000

0.11

Treatment

20

22.96**

152.72**

122.15**

1573.66**

9.25**

0.0005**

0.30**

Error

40

2.17

22.71

16.96

298.23

2.65

0.0001

0.16

Table 2: Phenotypic correlation


Characters

Plant Height

Plant seudostem height

Number of leaf

Leaf length

Leaf Width (mm)

Plant Seudostem diameter

Equatorial Diameter of bulbs

Polar Diameter

No of cloves /bulb

Weight of fresh bulbs

Weight of Dry bulbs

Yield quintal/ha

Total soluble solids %

Sulphur content %

Protein %

Plant Height

1.000

0.050

0.138

0.459**

0.131

0.200

0.440**

0.313*

0.003

0.562**

0.455**

0.008

-0.030

-0.246*

-0.047

Plant seudostem height

 

 

-0.567**

-0.008

-0.319**

-0.095

-0.148

-0.484**

0.171

-0.146

-0.205

0.032

-0.273*

0.157

-0.159

Number of leaf

 

 

 

0.152

0.708**

0.436**

0.364**

0.541**

0.081

0.415**

0.478**

0.427**

0.247*

-0.076

0.188

Leaf length

 

 

 

 

0.284*

0.433**

0.261*

0.095

0.077

0.437**

0.377**

0.208

0.005

0.032

0.097

Leaf Width (mm)

 

 

 

 

 

0.583**

0.343**

0.425**

0.090

0.397**

0.411**

0.494**

0.436**

0.063

0.227

Plant Seudostem dia.

 

 

 

 

 

 

0.511**

0.382**

0.444**

0.673**

0.659**

0.594**

0.338**

0.100

0.348**

Equatorial Dia. of bulbs

 

 

 

 

 

 

 

0.370**

0.178

0.755**

0.764**

0.323**

-0.081

0.001

0.175

Polar Diameter

 

 

 

 

 

 

 

 

0.067

0.462**

0.493**

0.258*

0.222

-0.276*

0.212

No of cloves /bulb

 

 

 

 

 

 

 

 

 

0.287*

0.328**

0.362**

0.055

0.338**

0.296*

Weight of fresh bulbs

 

 

 

 

 

 

 

 

 

 

0.908**

0.450**

0.156

-0.131

0.320**

Weight of Dry bulbs

 

 

 

 

 

 

 

 

 

 

 

0.471**

0.047

-0.039

0.255*

Yield quintal/ha

 

 

 

 

 

 

 

 

 

 

 

 

0.300*

0.064

0.428**

Total soluble solids %

 

 

 

 

 

 

 

 

 

 

 

 

 

0.023

0.429**

Sulphur content %

 

 

 

 

 

 

 

 

 

 

 

 

 

 

0.223

Protein %

 

 

 

 

 

 

 

 

 

 

 

 

 

 

1.000

*, ** significant at 5% and 1% level, respectively

Table 3: Genotypic correlation


Characters

Plant height

Plant seudostem hieght

Number of leaf

Leaf length

Leaf Width (mm)

Plant Seudostem diameter

Equatarial Diameter of bulbs

Polar Diameter

No of cloves /bulb

Weight of fresh bulbs

Weight of Dry bulbs

Yield quantal/ha

Total soluble solids %

Sulphur content %

Protein %

Plant height

1.000

-0.074

0.077

0.504**

-0.001

0.205

0.608**

0.421**

-0.045

0.663**

0.595**

-0.079

-0.305*

-0.406**

-0.259*

Plant seudostem hieght

 

 

-0.889**

-0.044

-0.922**

-0.232

-0.215

-0.708**

0.347**

-0.305*

-0.354**

-0.170

-0.439**

0.326**

-0.481**

Number of leaf

 

 

 

0.153

1.031**

0.512**

0.348**

0.757**

0.030

0.437**

0.564**

0.526**

0.306*

-0.232

0.362**

Leaf length

 

 

 

 

0.201

0.582**

0.530**

0.258*

0.160

0.756**

0.744**

0.261*

-0.134

0.037

-0.008

Leaf Width (mm)

 

 

 

 

 

0.874**

0.524**

0.771**

0.282*

0.693**

0.846**

0.832**

0.463**

-0.065

0.585**

Plant Seudostem dia.

 

 

 

 

 

 

0.649**

0.484**

0.576**

0.823**

0.825**

0.833**

0.398**

0.119

0.694**

Equatarial Dia. of bulbs

 

 

 

 

 

 

 

0.483**

0.226

0.931**

0.947**

0.507**

-0.341**

-0.133

0.409**

Polar Diameter

 

 

 

 

 

 

 

 

0.087

0.585**

0.651**

0.467**

0.300*

-0.436**

0.530**

No of cloves /bulb

 

 

 

 

 

 

 

 

 

0.368**

0.470**

0.606**

0.092

0.490**

0.716**

Weight of fresh bulbs

 

 

 

 

 

 

 

 

 

 

0.961**

0.628**

0.045

-0.187

0.557**

Weight of Dry bulbs

 

 

 

 

 

 

 

 

 

 

 

0.694**

-0.087

-0.069

0.559**

Yield quantal/ha

 

 

 

 

 

 

 

 

 

 

 

 

0.534**

0.024

0.759**

Total soluble solids %

 

 

 

 

 

 

 

 

 

 

 

 

 

-0.100

0.819**

Sulphur content %

 

 

 

 

 

 

 

 

 

 

 

 

 

 

0.399**

Protein %

 

 

 

 

 

 

 

 

 

 

 

 

 

 

1.000

*, ** significant at 5% and 1% level, respectively

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Kilgori, M., Magaji, M., & Yakubu, A. (2007). Effect of plant spacing and date of planting on yield of two garlic (Allium sativum L.) cultivars in Sokoto, Nigeria. American-Eurasian J. Agril. & Environmental Sci. 2(2), 153-157.
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Tsega, Kassahun, Tiwari, Akhilesh, Woldetsadik, & Kebede, (2011). Genetic variability among bulb yield and yield related traits in Ethiopian garlic (Allium sativum L.) germ plasm. Pantnagar Journal of Research. 9(1), 61-66.
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