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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2021, Volume : 9, Issue : 1
First page : (293) Last page : (296)
Article doi: : http://dx.doi.org/10.18782/2582-2845.8596

Studies to Identify Genes for Fragrance in Rice (Oryza sativa L.) through Gene Specific Primers

Vinit Kumar*, Rajat Chaudhary,  Amar Deep, Piyush Malik, Ankit Kumar,
Anurag Mishra, Sonum Arya and Devi Singh
Department of Genetics and Plant Breeding 
Sardar Vallabhbhai Patel University of Agriculture & Technology, Meerut, Uttar Pradesh, India
*Corresponding Author E-mail: amardeeptrivedivv@gmail.com
Received: 11.12.2020 | Revised: 17.01.2021 | Accepted: 24.01.2021 

 ABSTRACT

Atrial was conducted at the Crop Research Centre, Sardar Vallabhbhai Patel University of Agriculture & Technology, Meerut, India. During Kharif season 2014. The plant materials used in the study was consisted of forty genotypes of Rice (Oryza sativa L.). They were obtained from molecular biology laboratory, Deptt. of Genetics and Plant Breeding Sardar Vallabhbhai Patel University of Agriculture & Technology, Meerut, India. The data were analyzed based on the presence of fragrant and non-fragrant alleles. We can use these marker systems in marker assisted selection for incorporating the fgr gene from fragrant varieties into non fragrant varieties. These results show that mutation in badh2 or fgr gene is not universal to all fragrant rices. It may be possible that mutation elsewhere in badh2 or fgr gene or some other gene could be the likely cause for the fragrance in these genotypes. Based on these results, it can be assumed that fragrance in these genotypes may be controlled by some gene other than fgr.
Out of 40 varieties used, only 16 varieties were determined fragrant by six gene specific primers used. The eleven varieties Basmati-386, Vallabh Basmati-22, CSR-30, PS-2, PUSA-1401, Vallabh Basmati-24, PUSA-1121, Vallabh Basmati-23, IRBB-116, and Vallabh Basmati-21 & HB-1 expressed conspicuous fragrance. All of these 11 varieties were determined fragrant by all the six molecular markers used. It indicated that sequences for fragrance were available at all six loci of fgr gene represented by the corresponding six molecular markers used presently. The varieties Pusa Basmati-1, Punjab Basmati-2, PUSA-1509, Ranbir Basmati, CSR-23 expressed relatively low level of fragrance depending upon the involvement of number of loci containing DNA sequences responsible for development of fragrance. Fragrance of rice varieties PUSA-1509, Ranbir Basmati, CSR-23 was determined by 4, 3 and 1 loci, respectively. 

Keywords: Identification of genes for fragrance in rice genotypes/varieties using gene specific markers and Molecular characterization of rice for fragrance.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Kumar, V., Chaudhary, R., Deep, A., Malik, P., Kumar, A., Mishra, A., Arya, S., & Singh, D. (2021). Studies to Identify Genes for Fragrance in Rice (Oryza sativa L.) through Gene Specific Primers, Ind. J. Pure App. Biosci. 9(1), 293-296. doi: http://dx.doi.org/10.18782/2582-2845.8596