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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2021, Volume : 9, Issue : 1
First page : (99) Last page : (107)
Article doi: : http://dx.doi.org/10.18782/2582-2845.8544

Isolation, Characterization and Identification of Efficient Phosphate Solubilizing Bacteria from Different Chickpea Growing Areas of Karnataka

Dakshayini, G.*, Anantha Rama, A., Arunkumar Pandit., Muthuraju, R., Narayanaswamy, B, and Nagaraju, K.
Dept. of Agricultural Microbiology, University of Agricultural Sciences,
GKVK, Bengaluru – 560 065, Karnataka, India
*Corresponding Author E-mail: dakshayinig1492@gmail.com
Received: 13.12.2020  |  Revised: 16.01.2021   |  Accepted: 24.01.2021 

 ABSTRACT

Chickpea is an important pulse crop extensively grown in different parts of the world. India is the largest producer of chickpea whereas in Karnataka northern Karnataka has larger area under chickpea cultivation compared to southern regions of Karnataka. Phosphorus is the key element required in the nutrition of plants next to the nitrogen and these days phosphatic fertilizers are becoming costly and less available. In this context, an attempt was made to isolate and characterize the efficient phosphate solubilizing bacteria (PSB) from different areas of south Karnataka. Four different soil samples were collected from Arasikere, Chamarajanagara, Mysore and Devanahalli. PSB were isolated using Pikovskaya’s media and total of twenty four isolates were obtained. Based on zone of solubilization ten isolates were selected for screening process and from the result of screening tests three best isolates were identified by their morphological and biochemical characteristics as described in Bergey’s Manual of Determinative Bacteriology. From 16SrRNA sequencing, the top three most efficient PSB isolates were identified as Bacillus licheniformis(ACP-3),  Arthrobacter sp. HPG166 (ACP-2) and Pseudomonas chlororaphis (CCP-2). “Bacillus licheniformis” was the best isolate which was further tested under field condition with different fertilizer levels of SSP and rock phosphate

Keywords: PSB, Isolation, Characterization, Identification

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Dakshayini, G, Anantha Rama, A., Arunkumar P., Muthuraju, R., Narayanaswamy, B., & Nagaraju, K. (2021). Isolation, Characterization and Identification of Efficient Phosphate Solubilizing Bacteria from Different Chickpea Growing Areas of Karnataka, Ind. J. Pure App. Biosci. 9(1), 99-107. doi: http://dx.doi.org/10.18782/2582-2845.8544