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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2020, Volume : 8, Issue : 6
First page : (598) Last page : (610)
Article doi: : http://dx.doi.org/10.18782/2582-2845.7757

Development of Finger Millet (Eleusine coracana (L.) Gaertn.) Transgenic for Salt Tolerance by Overexpressing Antiporter Gene OsSOS1 Involved in Sodium Extrusion

Pushpa B. N.^1*, Kiranmai Kurnool² and A. G. Shankar^3
1Ph,D Scholar, ²Post doctoral fellow, ³Professor,
University of Agricultural Sciences, Bengaluru  
*Corresponding Author E-mail: pushpalatha0001@gmail.com
Received: 15.06.2020  |  Revised: 29.07.2020  |  Accepted: 10.08.2020  

 ABSTRACT

Salinity stress is one of the abiotic stress that adversely affect the plant growth and development. To maintain growth and productivity under salt stress conditions plants must exercise specific tolerant mechanisms. The OsSOS1 plasma membrane gene plays a crucial role in sodium extrusion from root epidermal cells under salinity. To develop salinity tolerant finger millet (GPU28) plants, OsSOS1 gene from Oryza sativa, was overexpressed by Agrobacterium tumerfaciens – mediated transformation. Direct plant regeneration by culturing shoot apical meristems (SAMs) was employed to generate transgenic plants as it is a simple and promising tool for plant regeneration because of rapid and effective regeneration capacity. The functional significance of OsSOS1 gene in transgenic plants (T1) was demonstrated invitro and also by invivo using Hydroponics system. Overexpressing the OsSOS1 gene in finger millet conferred high salt tolerance, promoted seed germination and increased root length, shoot length, chlorophyll, membrane stability index and reduction in reactive oxygen species (ROS) relative to wild type plants. These results clearly demonstrate that transgenic rice plants overexpressing OsSOS1, plasma membrane Na+/H+ antiporter have better salt-tolerance. This could have been mediated by the proton gradient generated by SOS1, which drives Na+/H+ antiporter which carries out extrusion of excess Na+ from cytosol into the apoplast and there by reducing the toxic effect of Na+ in the cell.

Keywords: Salinity, OsSOS1, SAMs, Transgenic plants

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Pushpa B. N, Kurnool, K. & Shankar, A. G. (2020). Development of Finger Millet (Eleusine coracana (L.) Gaertn.) Transgenic for Salt Tolerance by Overexpressing Antiporter Gene OsSOS1 Involved in Sodium Extrusion, Ind. J. Pure App. Biosci. 8(6), 598-610. doi: http://dx.doi.org/10.18782/2582-2845.7757