Archives

Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2020, Volume : 8, Issue : 5
First page : (421) Last page : (428)
Article doi: : http://dx.doi.org/10.18782/2582-2845.8347

In Vitro Propagation of Grape (Vitis vinifera L.) cv. Thompson Seedless

Said Ahmad Asim Hashemi^1* , B. N. Sathyanarayana² and Zarir Sharaf^3
1Department of Horticulture and Forestry, Agriculture Faculty, Baghlan University,
Baghlan Province, Afghanistan
²Department of Horticulture, University of Agricultural Sciences, GKVK, Bangalore, Karnataka, India
³Department of Horticulture and Forestry, Agriculture Faculty, Balkh University, Balkh Province, Afghanistan
*Corresponding Author E-mail: hashemi.hort@gmail.com
Received: 25.07.2020 | Revised: 26.09.2020 | Accepted: 1.10.2020 

 ABSTRACT

In vitro propagation of Vitis offers opportunities for increasing plant material for cultivation. Explant sterilization was accomplished by washing with 0.02 per cent Active-80 for 10 minutes followed by treating for 15 minutes in a solution containing 1000 ppm Bavistin + 100 ppm Cetrimide and final surface sterilization using (0.1%) mercuric chloride for 5 minutes. Between two different explants such as nodal cuttings and petiole segments, the nodal cuttings showed the maximum response of 90 per cent towards shoot induction on half strength MS medium. While, only 10 per cent of petiole segments responded, in that only root induction was seen. Induction of multiple shoots was found to be highest (3.6) on media supplemented with 2 mg/l BAP in cv. Thompson Seedless. Rooting of microshoots was successfully achieved and was cent percent with 1 mg/l IBA. In the same treatment early root initiation, maximum number of primary roots, lengthy roots and maximum number of secondary roots were also recorded. The higher concentrations of IBA at 4 mg/l and above were found to be deleterious in its effect, in that white friable calli was observed instead of rooting. Among different hardening media, Coirpith, Sand, Vermicompost, Vermicompost: Perlite (1:1), Soil: Sand: Vermicompost (1:1:2), the highest survival percentage (90) was observed with coirpith media and none of the plantlets survived on a sand media.

Keywords: Thompson Seedless Grape, In vitro propagation, MS medium, Nodal segment, Shoot proliferation, Root initiation.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Hashemi, S. A. A., Sathyanarayana, B. N. & Sharaf, Z. (2020). In Vitro Propagation of Grape (Vitis vinifera L.) cv. Thompson Seedless, Ind. J. Pure App. Biosci. 8(5), 421-428. doi: http://dx.doi.org/10.18782/2582-2845.8347