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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2020, Volume : 8, Issue : 4
First page : (568) Last page : (584)
Article doi: : http://dx.doi.org/10.18782/2582-2845.8259

Evaluation of Synergistic Potential of Zinc Oxide Nanoparticles from Plant Sources in Combination with Antibiotics on Multiple Drug-Resistant Pseudomonas aeruginosa

Madhumita Ghosh Dastidar^1* and M. Razia^2
1Department of Microbiology, Vijaya College, Bangalore, India
²Department of Biotechnology, Mother Teresa Women’s University, Kodaikanal, India
*Corresponding Author E-mail: madhumita.dastidar@gmail.com
Received: 2.07.2020  |  Revised: 14.08.2020   |  Accepted: 20.08.2020 

 ABSTRACT

Pseudomonas aeruginosa is a multiple drug resistant (MDR) bacteria which shows resistant to different classes of antibiotics and it causes severe complications in humans as well as animals. Such an organism – Pseudomonas aeruginosa was isolated from the hospital environment. Drug resistance concerning different classes of antibiotics was tested. For the treatment of the same antibiotic-resistant Pseudomonas bacteria, an alternative eco-friendly biochemical was being searched. Green nanoparticles are known to have immense applications in the field of medicine and biology which are eco-friendly, safe and stable. The zinc oxide nanoparticles were synthesized from plant leaves of Pongamia pinnata, Parthenium hysterophorus, Clematis montana using Zn (NO3)2 hexahydrate salt. The nanoparticles were analysed by UV-visible spectroscopy (UV–Vis), Scanning electron microscopy (SEM), Transmission Electron Microscopy (TEM), Energy Dispersive Analysis Of X-Ray (EDAX),Fourier Transform Infrared Spectroscopy(FTIR) and X-ray Diffraction( XRD) methods. It was observed that these nanoparticles acted as strong microbicidal agents against antibiotic-resistant Pseudomonas aeruginosa. Synergistic effects of antibiotic with zinc oxide nanoparticles were also observed in Pseudomonas aeruginosa and found to be effective. Thus, Zinc oxide nanoparticles (ZnONPs) in combination with antibiotics could be an interesting tool to control multiple antibiotic-resistant Pseudomonas aeruginosa.

Keywords: MDR, Antibiotics, Pseudomonas aeruginosa, Green Nanoparticles, Zinc oxide Nanoparticles

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Dastidar, M.G, & Razia, M. (2020). Evaluation of Synergistic Potential of Zinc Oxide Nanoparticles from Plant Sources in Combination with Antibiotics on Multiple Drug-Resistant Pseudomonas aeruginosa, Ind. J. Pure App. Biosci. 8(4), 568-584. doi: http://dx.doi.org/10.18782/2582-2845.8259