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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2020, Volume : 8, Issue : 4
First page : (267) Last page : (281)
Article doi: : http://dx.doi.org/10.18782/2582-2845.8261

Karyological and Genetic Diversity Study Using Molecular Marker among Three Species of Oilseed Brassica L.

Faria Akbar , Madhury Paul and Kazi Nahida Begum*
Department of Botany, Faculty of Life and Earth Science,
Jagannath University, Chittaranjan Avenue, Dhaka-1100, Bangladesh
*Corresponding Author E-mail: kazinahida@yahoo.com
Received: 17.06.2020  |  Revised: 23.07.2020   |  Accepted: 29.07.2020 

 ABSTRACT

Brassica campestris var. Agrani, B. juncea var. BARI Sharisha-16 (BS-16) and B. napus var. BARI Sharisha-13 (BS-13) are the three different oilseed crops belonging to the genus Brassica, have been investigated to amass genetic information through cytogenetic characterization by orcein staining and to unveil phylogenetic relationships among these three species by karyomorphological analyses with respect to their asymmetry indices. B. campestris var. Agrani, B. juncea var. BS-16 and B. napus var. BS-13 were found to possess different somatic chromosome number along with varied karyotype formulae, 2n=20=20m, 2n=36=30m+6sm and 2n=38=34m+4sm, respectively. The investigation also aimed at exploring the genetic divergence and selection of improved variety among the three Brassica species by protein banding pattern obtained from polyacrylamide gel electrophoresis (PAGE). According to Nei’s genetic distance analysis, the highest genetic distance found between B. juncea var. BS-16 was 0.4700. Contrastingly, the lowest genetic distance found between B. campestris var. Agrani and B. napus var. BS-13 was 0.2877. B. juncea var. BS-16 was found at distant position in the phylogenetic tree and may displayed diverse nature from other two species of Brassica L. The values of different karyomorphological indices also presaged that B. juncea var. BS-16 exhibited some degree of asymmetry and may express with relatively advanced nature than the other two studied species in respect of evolutionary perspective. Therefore, this study regarding detailed karyomorphological data and determination of genetic relationship would be beneficial to the breeders of Brassica for proper characterization, designing upcoming breeding programs and conservation purposes of plant genetic resources of Brassica variants.

Keywords:Karyotype, Asymmetry, PAGE, Brassica L.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Akbar, F., Paul, M., & Nahida Begum, K. (2020). Karyological and Genetic Diversity Study Using Molecular Marker among Three Species of Oilseed Brassica L., Ind. J. Pure App. Biosci. 8(4), 267-281. doi: http://dx.doi.org/10.18782/2582-2845.8261