Archives

Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2020, Volume : 8, Issue : 4
First page : (172) Last page : (183)
Article doi: : http://dx.doi.org/10.18782/2582-2845.8254

Molecular Characterization of Begomovirus Infecting Black Gram (Vigna mungo L.) from East Godavari, Andhra Pradesh

D. Vijay Kumar Naik^1* , B. V. Bhaskara Reddy², R. Sarada Jayalakshmi Devi¹, L. Prasanthi² ,V. Lakshmi Narayana Reddy¹ and A. Srividhya^2
1S. V. Agriculture College, Acharya N. G. Ranga Agricultural University, Tirupati
²Regional Agricultural Research Station, Acharya N. G. Ranga Agricultural University, Tirupati
*Corresponding Author E-mail: dvnaik24@gmail.com
Received: 5.07.2020  |  Revised: 3.08.2020   |  Accepted: 8.08.2020 

 ABSTRACT

For molecular characterization of Yellow Mosaic Virus (YMV) associated with Yellow Mosaic Disease (YMD) of blackgram, infected blackgram samples were collected from farmer field of East Godavari district, Andhra Pradesh. The total DNA was isolated by CTAB method, amplified in PCR with coat protein and movement protein gene specific primers. The resulting PCR products were sequenced and submitted to NCBI GenBank. Comparison of coat protein and movement protein nucleotide sequence with other begomoviruses collected from NCBI GenBank revealed that DNA-A of MYMIV and MYMV of present isolate shared 99.3% and 97.2% similarity with MYMIV-Raichur (MN698280) and MYMV-Vamban (KC911722) isolates respectively. MYMV DNA-B of present isolate shared 96% identity with MYMV-Tirupati (KF947526) isolate. Phylogenetic tree based on coat protein gene nucleotide sequence of MYMIV, MYMV and movement protein gene nucleotide sequence of MYMV infecting East Godavari isolate with other isolates collected from NCBI GenBank formed unique clusters with DNA-A of MYMIV, MYMV and DNA-B of MYMV respectively. The results revealed that both MYMIV and MYMV are associated with YMD of blackgram in East Godavari district of Andhra Pradesh.

Keywords: Begomoviruses, Coat protein, Movement protein, Characterization, Nucleotide sequence similarity, East Godavari isolate, Phylogenetic tree.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Naik, D.V.K., Reddy, B.V.B., Jayalakshmi Devi, R.S., Prasanthi, L., Reddy, V.L.N., & Srividhya, A. (2020). Molecular Characterization of Begomovirus Infecting Black Gram (Vigna mungo L.) from East Godavari, Andhra Pradesh, Ind. J. Pure App. Biosci. 8(4), 172-183. doi: http://dx.doi.org/10.18782/2582-2845.8254