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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2020, Volume : 8, Issue : 3
First page : (37) Last page : (47)
Article doi: : http://dx.doi.org/10.18782/2582-2845.8096

RAPD Analysis of Genetic Diversity and Relationships among Kenaf (Hibiscus cannabinus L.) Germplasm

Mohammad Golam Mostofa* , Lutfur Rahman, Abu Saleh Muhammad Yahiya, Mohammad Harun-or-Rashid and Mohammad Mukul Mia
Breeding Division, Bangladesh Jute Research Institute, Manik Mia Avenue, Dhaka-1207, Bangladesh
*Corresponding Author E-mail: mostofabjri@gmail.com
Received: 15.04.2020  |  Revised: 21.05.2020   |  Accepted: 27.05.2020 

 ABSTRACT

To analyse the genetic diversity and relationships among kenaf germplasm, two cultivars and twenty three accessions from different geographical regions were assessed using RAPD markers. Six RAPD primers generated 38 polymorphic bands with an average of 6.33 polymorphic bands per primer. A high level of genetic variation (proportion of polymorphic loci 79.17%) and high genetic distance (0.0426 to 0.6523) were found among 25 indigenous and exotic kenaf germplasm. The UPGMA dendrogram based on Nei’s genetic distance segregated the genotypes into two major, two minor and one single genotype clusters. The distribution pattern of genotypes from different geographical regions into five clusters was random, indicating that geographical isolation may not be only factors causing genetic diversity. The minor cluster III comprised two accessions collected from same origin but they are distantly related to each other. The genetic distance between two cultivars was lower (0.0870) and they were found in the same cluster. These results indicate that studied kenaf germplasm not only exhibit a high level of genetic diversity but also have a different genetic background.  

Keywords: Diversity, Germplasm, RAPD, Kenaf

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Mostofa, M.G., Rahman, L., Muhammad Yahiya, A.S., Harun-or-Rashid, M., & Mukul Mia, M. (2020). RAPD Analysis of Genetic Diversity and Relationships among Kenaf (Hibiscus cannabinus L.) Germplasm, Ind. J. Pure App. Biosci. 8(3), 37-47. doi: http://dx.doi.org/10.18782/2582-2845.8096