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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2020, Volume : 8, Issue : 3
First page : (1) Last page : (12)
Article doi: : http://dx.doi.org/10.18782/2582-2845.8075

Molecular Identification of Date Palm Varieties Using Chloroplast Barcode atpF-atpH Spacer

Mohamed R. Enan^1,3* and Salah Ali Moustafa^1,2
1Agricultural Research Center, Agricultural Genetic Engineering Research Institute, Giza, Egypt
²Biology Department, Faculty of Science, Jazan University, Saudia Arabia
³Biology Department, Faculty of Science, United Arab Emirates University, United Arab Emirates
*Corresponding Author E-mail: mohamed.enan@uaeu.ac.ae
Received: 16.04.2020  |  Revised: 22.05.2020   |  Accepted: 27.05.2020 

 ABSTRACT

DNA barcoding is a technique for discriminating and identifying species using short, variable, and standardized DNA regions. Here, we tested for the first time the performance of chloroplast atpF-atpH spacer as DNA barcodes in Phoenix dactylifera varieties.  The lack of differential morphological and anatomical useful characters, and interspecific hybridization, make identification of Phoenix species difficult. In this context, the development of reliable DNA markers for varieties identification would be of great utility.  therefore, the present study aimed at the evaluation of genetic relationship based on chloroplast atpF-atpH spacer was amplified and sequenced from selected varieties. Phylogram illustrated over all genetic distance of 0.0002 representing close genetic relationship of selected P. dactylifera varieties. Pairwise distance was calculated for atpF-atpH spacer and very low genetic diversity value was observed (0.002). Estimates of average evolutionary divergence of overall sequence pairs and nucleotide diversity were again found very low with 0.008. Based on atpF-atpH genetic makeup, it can be suggested that date palm varieties show very high degree of similarity.

Keywords: Chloroplast atpF-atpH regions, DNA barcode, Phoenix dactylifera

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Enan, M.R., & Moustafa, S.A. (2020). Molecular Identification of Date Palm Varieties Using Chloroplast Barcode atpF-atpH Spacer, Ind. J. Pure App. Biosci. 8(3), 1-12. doi: http://dx.doi.org/10.18782/2582-2845.8075