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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2020, Volume : 8, Issue : 2
First page : (377) Last page : (388)
Article doi: : http://dx.doi.org/10.18782/2582-2845.8047

Biochemical Basis of Systemic Acquired Resistance Induced by Different SAR Elicitors against Late Blight of Potato

Astha* and Sekhon P. S.
Department of Plant Pathology, PAU, Ludhiana-141004
*Corresponding Author E-mail: astha-asr@pau.edu
Received: 11.03.2020  |  Revised: 17.04.2020   |  Accepted: 21.04.2020 

 ABSTRACT

Phytophthora infestans is an oomycete that causes a serious potato disease known as late blight of potato. The present study was conducted to reduce fungicide load and work out alternate method for control of this disease.  Different SAR compounds were tested and exogenous foliar sprays of  different conc. of Salicylic acid,  Jasmonic acid and Bion (Benzothiadiazole-BTH) @ 50µM, 250µM, 500µM, 1000µM and of β- amino butyric acid of 20 mM, 30mM, 50 mM, 100mM were given for inducing resistance in potato against late blight disease. Concentration of Salicylic acid, Jasmonic acid and Bion @ 500 µM, and β- amino butyric acid @ 50 mM gave best control of disease among all tested concentrations. Protein content of treated potato plant varied from 4.3 to 8.1 mg/g fresh weight compared to 3.2 mg/g fresh weight in control. Induction of proteins and defense enzymes was systemic in nature in response to all the four elicitors. The inducers also stimulated  the activities of pathogenesis related proteins (Pr- proteins) i.e. β-1,3 glucanase, Peroxidase (POD), and defense related proteins i.e. Polyphenol oxidase (PPO), Phenylalanine ammonia lyase (PAL) from 26 to 99 % indicating induced resistance in treated potato plants as compared to control. Electrophoretic protein profiling of treated potato plants also confirmed the induction of pathogenesis-related proteins ranging from 15- 75 kDa along with some other proteins. Total chlorophyll and carotenoids also showed spike of 1% to 137 % in response to elicitors. Salicylic acid gave best results with 82.6 % disease control followed by Jasmonic acid with 79.2%; whereas Bion and Βeta amino butyric acid were almost at par with each other and gave 75 % disease control as compared to control plants. Thus integration of disease tolerance and salicylic acid spray resulted in effective and eco-friendly control of late blight of potato.

Keywords: Potato, Systemic acquired resistance, Salicylic acid, Jasmonic acid, β-amino butyric acids (BABA), Benzothiadiazole (BTH), Late blight.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Astha, & Sekhon, P.S. (2020). Biochemical Basis of Systemic Acquired Resistance Induced by Different SAR Elicitors against Late Blight of Potato, Ind. J. Pure App. Biosci. 8(2), 377-388. doi: http://dx.doi.org/10.18782/2582-2845.8047