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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2020, Volume : 8, Issue : 2
First page : (219) Last page : (223)
Article doi: : http://dx.doi.org/10.18782/2582-2845.8025

TILLING - Reverse Genetics Technique for Identification of Mutation in Plants

Khushbu Chittora*
Department of Genetics and Plant Breeding, Rajasthan College of Agriculture,
MPUAT, Udaipur, Rajasthan-313001
*Corresponding Author E-mail: chittorakhushbu23@gmail.com
Received: 8.03.2020  |  Revised: 13.04.2020   |  Accepted: 17.04.2020 

 ABSTRACT

TILLING is widely used in plant functional genomics. Mutagenesis and SNP detection is combined to allow for the isolation of mutations in genes of interest. It can also be used as a plant breeding tool, whereby variation in known or candidate genes of interest to breeding programs is generated. Here we describe a simple low-cost TILLING procedure.
            Tilling is a reverse genetic strategy which mutation is induced and followed by high throughput screening of point mutation in gene of interest. TILLING provides a powerful approach for gene discovery, DNA polymorphism assessment, and plant improvement. It is a rapid and low-cost method. Coupled with other genomic resources, TILLING and EcoTILLING can be used immediately as a haplotyping tool in crop improvement for identifying allelic variation in genes exhibiting expression correlating with phenotypes.

Keywords: Reverse genetics, TILLING, Mutation, EMS,

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Chittora, K. (2020). TILLING - Reverse Genetics Technique for Identification of Mutation in Plants, Ind. J. Pure App. Biosci. 8(2), 219-223. doi: http://dx.doi.org/10.18782/2582-2845.8025