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Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2019, Volume : 7, Issue : 5
First page : (43) Last page : (49)
Article doi: : http://dx.doi.org/10.18782/2320-7051.7804

miRNA-21 Signature in the Plasma Samples of Patients with Extrapulmonary Tuberculosis

Anmol Kulshrestha¹, Aditya Upadhyay¹, Malti Dadheech⁴, R.R.K. Niraj², Deepika Gupta⁴, Suresh K. Jatawa¹, Archana Tiwari¹, Jitendra K. Sharma³, Shubhra Jain³ and
Sandeep K. Shrivastava^2,4,*
1School of Biotechnology – Rajiv Gandhi Proudyogiki Vishwavidyalaya, Airport Road, Bhopal, 462033, India
²Dr. B. Lal Institute of Biotechnology, Jaipur, India
³Department of Respiratory Medicine, Sawai Man Singh (SMS) Medical College, Jaipur, 302004, India
⁴Centre for Innovation, Research and Development (CIRD),
Dr. B. Lal Clinical Laboratories Pvt. Ltd., Jaipur, 302017, India
*Corresponding Author E-mail: ssk.cird1@gmail.com
Received: 22.08.2019  |  Revised: 30.09.2019   |  Accepted: 8.10.2019  

 ABSTRACT

Tuberculosis is an infectious disease causing more than 1.6 million deaths worldwide every year. This morbidity is due to the lack of early diagnosis of tuberculosis especially in the Extrapulmonary Tuberculosis. In this study, we assess the regulation of miRNA-21 in the plasma samples of Extrapulmonary Tuberculosis infected patients. This study employed 20 Extrapulmonary Tuberculosis plasma samples and 10 Healthy control plasma samples. After evaluating the expression of miRNA-21 by Quantitative Real-Time PCR [qRT-PCR], miRNA-21 was found to be up-regulated in plasma samples of Extrapulmonary Tuberculosis as compared to the plasma samples of Healthy control. The expression analysis was mainly done by using 2-ΔΔCT method. The ROC curve analysis revealed the diagnostic potential of the miRNA-21 in Extrapulmonary Tuberculosis with a good AUC value of 0.95. Our results conclude that the miRNA-21 have a potential application and can act as a biomarker in the diagnosis of Extrapulmonary Tuberculosis.

Keywords:  Pulmonary tuberculosis, Extrapulmonary tuberculosis, Circulating miRNA-21, Biomarker, Up-regulation, Stop TB Program, ROC

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Kulshrestha, A., Upadhyay, A., Dadheech, M., Niraj, R.R.K., Gupta, D., Jatawa, S.K., Tiwari, A., Sharma, J.K., Jain, S. and Shrivastava, S.K.  (2019). miRNA-21 Signature in the Plasma Samples of Patients with Extrapulmonary Tuberculosis, Ind. J. Pure App. Biosci.7(5), 43-49. doi: http://dx.doi.org/10.18782/2320-7051.7804