Archives

Indian Journal of Pure & Applied Biosciences (IJPAB)
Year : 2019, Volume : 7, Issue : 4
First page : (1) Last page : (7)
Article doi: : http://dx.doi.org/10.18782/2320-7051.7641

Molecular Identification of Azo Dye Degrading Fungi Isolated from Azo Dye Contaminated Soil of Local Dyeing Facility in Bida, Niger State

Abubakar Hassana¹*, Balogu Tochukwu Vincent², Isah Muhammad Nasiru¹, Ndatsu Yakubu¹, Mayaki Fatima Gogo¹ and Usman Hamza Boko^1
1Department of Biochemistry, ²Department of Microbiology,
Ibrahim Badamasi Babangida University, Lapai, Nigeria
*Corresponding Author E-mail: ahassana@ibbu.edu.ng
Received: 5.06.2019 | Revised: 12.07.2019 | Accepted: 18.07.2019  

 ABSTRACT

Azo dyes are synthetic organic colorants containing one or more azo bonds (–N=N). These dyes constitute the major chemical in textile industrial waste water and are released into the ecosystem. Biotransformation of these azo compounds produces intermediates like aromatic amines which are very carcinogenic and mutagenic. Several physicochemical methods used in the treatment of these azo compounds are usually ineffective, expensive, and not always applicable. This study aimed at isolating and identifying azo dye degrading fungi from azo dye contaminated soil taken from a local dyeing facility in Bida local government area, Niger State, Nigeria. Isolation was done on mineral salt medium containing 1% congo red azo dye as carbon and energy source. The isolates were characterized based on microscopic and culture morphology and identified with molecular characterization. Degradation potential of the isolates were determined in mineral salt broth with 1% congo red dye as carbon source using UV spectrophotometer at wave length of 590 nm. The isolate with the highest degradation rate was further identified using ITS Gene Seguence. Two fungi were isolated; MSN1 and SN3. MSN1 was identified as Aspergillus fumigatus while SN3 was Penicellium chrysogenum.Aspergillus fumigatus attained the highest degradative activity of 0.93 Optical Density on the sixth day and Penicellium chrysogenum highest degradative activity was 0.70 Optical Density on the ninth day. ITS Gene Seguencing of Aspergillus fumigatus confirmed that this fungi belongs to this species with 99% similarity. This specie is known to have great dye decolorizing ability, hence its application in dye bioremediation is highly recommended.

Keywords: Azo dye, congo red, degradation, Aspergillus fumigatus.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Hassana, A., Vincent, B.T., Nasiru, I.M., Yakubu, N., Gogo, M.F. & Boko, U.H. (2019). Molecular Identification of Azo Dye Degrading Fungi Isolated from Azo Dye Contaminated Soil of Local Dyeing Facility in Bida, Niger State, Ind. J. Pure App. Biosci. 7(4), 1-7. doi: http://dx.doi.org/10.18782/2320-7051.7641