Archives

International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2019, Volume : 7, Issue : 2
First page : (34) Last page : (38)
Article doi: : http://dx.doi.org/10.18782/2320-7051.7366

Effect of Various pH Values on the Efficacy of Phosphate Solubilising Microbes Isolated from Rhizosphere of Rose and China Rose

Arushi Makkar¹, Anshu Sibbal Chatli²*, Parneet Kaur¹, Navdeep Kaur¹ and Neha Gupta³

¹P.G. Students, Department of Biotechnology,
²*Assistant Professor, Department of Microbiology,
³Assistant Professor, Department of Biotechnology,
Guru Nanak Girls College, Model Town, Ludhiana (Punjab)-141002, India
*Corresponding Author E-mail: manansh@hotmail.com
Received: 18.02.2019 | Revised: 22.03.2019 | Accepted: 27.03.2019  

 ABSTRACT

Phosphate Solubilising Microbes (PSMs) were isolated and screened from the rhizosphere of Rosa and Hibiscus rosa sinensis. These microbes were tested to see their potentials to solubilise Tricalcium Phosphate (TCP) at different pH values (pH 4, 6, 7, 9 and 11) on Pikovskaya (PVK) agar media and broth. It was observed that bacterium (Micrococcus) showed its maximum efficacy to solubilse TCP under neutral to alkaline conditions while fungi under neutral to acidic conditions. Of the total PSF found, Non sporulating sterile formed biggest halo zone (12.2 mm) followed by Penicillium sp. (11 mm) and Aspergillus niger (7.3 mm) on PVK agar media. The Non sporulating sterile Fungus also dissolved maximum TCP (28.5 μg/ml) in PVK broth and decreased the pH of filterate (3.2) at pH 6 of media. Phosphate solubilising bacterium Micrococcus showed its maximum halo size (8.1 mm) and TCP dissolution (27.3 μg/ ml) at pH 9. This bacterium decreased the pH of filtrate to 6.4 at pH 9 of PVK broth.

Key words: PVK, TCP, Phosphate solubilising microbes, pH values.

Full Text : PDF; Journal doi : http://dx.doi.org/10.18782

Cite this article: Makkar, A., Chatli, A.S., Kaur, P., Kaur, N. and Gupta, N., Effect of Various pH Values on the Efficacy of Phosphate Solubilising Microbes Isolated from Rhizosphere of Rose and China Rose, Int. J. Pure App. Biosci.7(2): 34-38 (2019). doi: http://dx.doi.org/10.18782/2320-7051.7366