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International Journal of Pure & Applied Bioscience (IJPAB)
Year : 2019, Volume : 7, Issue : 2
First page : (154) Last page : (161)
Article doi: : http://dx.doi.org/10.18782/2320-7051.7377
Study of Molecular Diversity in High Zinc Rice (Oryza sativa L.) Genotypes
Ambily Elizabath*, Midhun Babychan1, Annie Merly Mathew2 and Golda Maria Syriac3
Department of Genetics and Plant Breeding, Institute of Agricultural Sciences,
Banaras Hindu University, Vanarasi, India
*Corresponding Author E-mail: sonali.habde@gmail.com
Received: 27.02.2019 | Revised: 30.03.2019 | Accepted: 7.04.2019
ABSTRACT
Assessment of genetic diversity is a key factor in germplasm characterization and conservation. An investigation was carried out with 20 rice genotypes constituting checks collected from different sources using 13 microsatellite markers to study the nature and magnitude of genetic divergence. The results showed that all the SSRs were polymorphic with different alleles among the genotypes studied showing the robust nature of microsatellites in revealing polymorphism. The average PIC value was 0.349 and it ranged from a low of 0.090 (RM OSR) to a high of 0.572 (RM 495). The Jaccard’s similarity coefficient ranged from 0.231 to 1. The UPGMA dendrogram revealed two major groups with five clusters and the wide range of dissimilarity values (0.01-0.88) which represents a high degree of diversity among the genotypes. The results of the genetic diversity can be exploited for the selection of the parents for developing high zinc rice variety through molecular breeding program.
Key words: Microsatellites, Polymorphism, PIC value, UPGMA dendrogram.
Full Text : PDF; Journal doi : http://dx.doi.org/10.18782
Cite this article: Sharma, Y., Singh, S.K., Vennela, P.R., Dey, R., Singh, D.K. and Habde, S. Study of Molecular Diversity in High Zinc Rice (Oryza sativa L.) Genotypes, Int. J. Pure App. Biosci.7(2): 154-161 (2019). doi: http://dx.doi.org/10.18782/2320-7051.7377